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Literature summary for 1.1.1.27 extracted from

  • Terlecki, G.; Czapi?ska, E.; Hotowy, K.
    Ultracentrifugation studies of the location of the site involved in the interaction of pig heart lactate dehydrogenase with acidic phospholipids at low pH. A comparison with the muscle form of the enzyme (2007), Cell. Mol. Biol. Lett., 12, 378-395.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.27

Inhibitors

Inhibitors Comment Organism Structure
additional information NADH, NAD+, ATP, ADP, AMP, and pyruvate inhibit the interaction of the heart-type isozyme with acidic phospholipid liposomes, potency in descending order. NADP+, GTP, CTP, UTP and lactate are ineffective, overview Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Sus scrofa
-
 pyruvate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Sus scrofa
-
heart heart-type isozyme Sus scrofa
-
skeletal muscle muscle-type isozyme, i.e. M4 isoform Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
 Sus scrofa pyruvate + NADH + H+
-
 r
additional information the heart-type isozyme interacts with liposomes made of acidic phospholipids, such as phosphatidylserine or cardiolipin, most effectively at low pH close to the isoelectric point of the isozyme of pH 5.5 strongly involving the enzyme's NADH-cofactor binding site, no interaction with liposomes of the muscle-type isozyme, overview Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
tetramer heart-type isozyme Sus scrofa

Synonyms

Synonyms Comment Organism
heart LDH
-
 Sus scrofa
lactate dehydrogenase
-
 Sus scrofa
LDH
-
 Sus scrofa
muscle LDH
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sus scrofa
NADH the NADH-cofactor binding site of heart LDH is involved in the interaction of the isozyme with liposomes made of acidic phospholipids, overview Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa heart-type isozyme
-
 5.5