Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Lacticaseibacillus casei | |
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Latilactobacillus curvatus |
General Stability | Organism |
---|---|
irreversible loss of activity after several hours when the concentration is below 0.1 mg protein per ml | Latilactobacillus curvatus |
Mn2+ and fructose 1,6-diphosphate are required during dialysis at pH 5.5, very unstable during dialysis at pH 7.5 although Mn2+ and fructose 1,6-diphosphate are added | Latilactobacillus curvatus |
stable to dilution in the range 0.2-0.01 mg protein per ml | Lacticaseibacillus casei |
very stable during 70 h dialysis in acetate buffer pH 5.5, in imidazol buffer, pH 6.5, in phosphate buffer pH 6.5 with dithioerythritol and in Tris-HCl buffer pH 7.5 with Mn2+ and fructose 1,6-diphosphate | Lacticaseibacillus casei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | at low pH-values | Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of pH | Lacticaseibacillus casei | |
additional information | - |
additional information | influence of pH | Latilactobacillus curvatus | |
280 | - |
(S)-lactate | - |
Latilactobacillus curvatus | |
420 | - |
(S)-lactate | - |
Lacticaseibacillus casei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation is as effective as with Mn2+, Co2+ and Cd2+ | Lacticaseibacillus casei | |
Ca2+ | 50% of the stimulation with Mn2+, Co2+ or Cd2+, higher concentrations required | Latilactobacillus curvatus | |
Cd2+ | stimulation | Lacticaseibacillus casei | |
Cd2+ | stimulation | Latilactobacillus curvatus | |
Co2+ | stimulation | Lacticaseibacillus casei | |
Co2+ | stimulation | Latilactobacillus curvatus | |
Mn2+ | stimulation | Lacticaseibacillus casei | |
Mn2+ | stimulation | Latilactobacillus curvatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
ssp. casein | - |
Latilactobacillus curvatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lacticaseibacillus casei |
- |
Latilactobacillus curvatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2030 | - |
- |
Latilactobacillus curvatus |
2320 | - |
- |
Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | the maximal velocity of lactate oxidation is about 10% of pyruvate reduction | Lacticaseibacillus casei | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | the maximal velocity of lactate oxidation is about 10% of pyruvate reduction | Latilactobacillus curvatus | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | the maximal velocity of lactate oxidation is only 10% of pyruvate reduction | Lacticaseibacillus casei | (S)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | the maximal velocity of lactate oxidation is only 10% of pyruvate reduction | Latilactobacillus curvatus | (S)-lactate + NAD+ | - |
r | |
pyruvate + NADPH + H+ | 20% of the activity with NADH | Latilactobacillus curvatus | (S)-lactate + NADP+ | - |
? | |
pyruvate + NADPH + H+ | 15% of the activity with NADH | Lacticaseibacillus casei | (S)-lactate + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
relatively stable | Lacticaseibacillus casei |
60 | - |
5 min, complete inactivation without stabilizer, Mn2+ in combination with fructose 1,6-diphosphate stabilizes the enzyme completely | Latilactobacillus curvatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Lacticaseibacillus casei |
4.5 | - |
- |
Latilactobacillus curvatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Lacticaseibacillus casei | |
NAD+ | coenzyme | Latilactobacillus curvatus | |
NADH | coenzyme | Lacticaseibacillus casei | |
NADH | coenzyme | Latilactobacillus curvatus | |
NADPH | coenzyme | Lacticaseibacillus casei | |
NADPH | coenzyme | Latilactobacillus curvatus |