Cloned (Comment) | Organism |
---|---|
recombinant expression | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0098 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
0.115 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
53 | - |
1-deoxy-L-erythrulose | pH 7.5, 25°C, recombinant enzyme in absence or presence of phosphite dianion | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Mycobacterium tuberculosis | |
phosphite dianion | activation by phosphite dianion | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | random ordered mechanism, though binding of NADPH first is preferential at 0.2 mM due to its low Km. Conformational equilibrium between a predominant, inactive open form and a minor, active closed form of the unliganded enzyme, overview | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-L-erythrulose + NADPH + H+ | reaction of the truncated substrate 1-deoxy-L-erythrulose in the presence and absence of phosphite dianion, overview | Mycobacterium tuberculosis | ? + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DXP reductoisomerase | - |
Mycobacterium tuberculosis |
DXR | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
1-deoxy-L-erythrulose | pH 7.5, 25°C, recombinant enzyme in presence of phosphite dianion | Mycobacterium tuberculosis | |
0.00155 | - |
1-deoxy-L-erythrulose | pH 7.5, 25°C, recombinant enzyme in absence of phosphite dianion | Mycobacterium tuberculosis | |
5.25 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | 1-deoxy-D-xylulose-5-phosphate, DXP, reductoisomerase catalyzes the first committed step in the 2-C-methyl-D-erythritol 4-phosphate pathway involving a skeletal rearrangement of DXP via a retroaldol/aldol process followed by reduction by NADPH to yield 2-C-methyl-D-erythritol 4-phosphate | Mycobacterium tuberculosis |
additional information | role of the nonreacting phosphorylmethyl group in catalysis, overview | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000292 | - |
1-deoxy-L-erythrulose | pH 7.5, 25°C, recombinant enzyme in absence of phosphite dianion | Mycobacterium tuberculosis | |
0.000163 | - |
1-deoxy-L-erythrulose | pH 7.5, 25°C, recombinant enzyme in presence of phosphite dianion | Mycobacterium tuberculosis | |
46 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis |