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Literature summary for 1.1.1.267 extracted from

  • Rohdich, F.; Lauw, S.; Kaiser, J.; Feicht, R.; Koehler, P.; Bacher, A.; Eisenreich, W.
    Isoprenoid biosynthesis in plants - 2C-methyl-D-erythritol-4-phosphate synthase (IspC protein) of Arabidopsis thaliana (2006), FEBS J., 273, 4446-4458.
    View publication on PubMed
Search for more literature for 1.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
gene ispC, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in the pseudomature form without plastid-targeting sequence in recombinant Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
fosmidomycin
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADPH pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
0.132
-
 1-deoxy-D-xylulose 5-phosphate pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
0.47 1 NADP+ pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
0.972
-
 2-C-methyl-D-erythritol 4-phosphate pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ a divalent cation is absolutely required, optimal at 10 mM, the enzyme prefers Mn2+ or Mg2+ Arabidopsis thaliana
Mn2+ a divalent cation is absolutely required, the enzyme prefers Mn2+ or Mg2+ Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Arabidopsis thaliana second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9XFS9 DXR; gene ispC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme in the pseudomature form without plastid-targeting sequence 62fold from Escherichia coli Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
 purified recombinant pseudomature enzyme, reverse reaction Arabidopsis thaliana
5.6
-
 purified recombinant pseudomature enzyme, forward reaction Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, 56% remaining activity Arabidopsis thaliana
-80°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, no loss of activity Arabidopsis thaliana
4°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, 4% remaining activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ the forward reaction is preferred Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+
-
 r
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Synonyms

Synonyms Comment Organism
IspC
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 42 90% of maximal activity within this range Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purifed recombinant enzyme, 24 h, 78% remaining activity Arabidopsis thaliana
37
-
 purifed recombinant enzyme, 24 h, 30% remaining activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 NADP+ pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
1.6
-
 2-C-methyl-D-erythritol 4-phosphate pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
4.4
-
 NADPH pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana
4.4
-
 1-deoxy-D-xylulose 5-phosphate pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 maximally stable at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana
NADH 14% of the activity with NADPH Arabidopsis thaliana
NADP+
-
 Arabidopsis thaliana
NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000085
-
 fosmidomycin pH 8.0, 37°C, recombinant pseudomature enzyme Arabidopsis thaliana