Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Ensifer adhaerens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion | Ensifer adhaerens |
Protein Variants | Comment | Organism |
---|---|---|
A13G | decreased activity | Ensifer adhaerens |
A13G/S10G | decreased activity | Ensifer adhaerens |
A13G/S33D | increased activity | Ensifer adhaerens |
D176A | decreased activity | Ensifer adhaerens |
G206I | decreased activity | Ensifer adhaerens |
H180A | decreased activity | Ensifer adhaerens |
K94G | decreased activity | Ensifer adhaerens |
S10G | increased activity | Ensifer adhaerens |
S33D | decreased activity | Ensifer adhaerens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | mutant A13G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.06 | - |
NADPH | mutant G206I in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.06 | - |
NADPH | recombinant wild type enzyme in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.1 | - |
NADPH | native wild type enzyme in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.2 | - |
NADPH | mutant K94G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.27 | - |
NADPH | mutant S10G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
0.38 | - |
NADPH | mutant A13G/S10G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
1 | - |
NADPH | mutant A13G/S33D in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
1.1 | - |
NADH | mutant A13G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
1.1 | - |
NADH | mutant A13G/S33D in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
1.2 | - |
NADH | mutant A13G/S10G in the presence of 1,5-anhydro-D-fructose | Ensifer adhaerens | |
3.2 | - |
1,5-Anhydro-D-fructose | mutant A13G/S33D in the presence of NADH | Ensifer adhaerens | |
3.5 | - |
1,5-Anhydro-D-fructose | mutant S10G in the presence of NADPH | Ensifer adhaerens | |
6.4 | - |
1,5-Anhydro-D-fructose | recombinant wild type enzyme in the presence of NADPH | Ensifer adhaerens | |
7.1 | - |
1,5-Anhydro-D-fructose | mutant A13G/S10Gin the presence of NADPH | Ensifer adhaerens | |
8.3 | - |
1,5-Anhydro-D-fructose | mutant G206I in the presence of NADPH | Ensifer adhaerens | |
8.3 | - |
1,5-Anhydro-D-fructose | native wild type enzyme in the presence of NADPH | Ensifer adhaerens | |
8.5 | - |
1,5-Anhydro-D-fructose | mutant A13G in the presence of NADPH | Ensifer adhaerens | |
8.9 | - |
1,5-Anhydro-D-fructose | mutant H108A in the presence of NADPH | Ensifer adhaerens | |
11.1 | - |
1,5-Anhydro-D-fructose | mutant A13G in the presence of NADH | Ensifer adhaerens | |
20.2 | - |
1,5-Anhydro-D-fructose | mutant A13G/S33D in the presence of NADPH | Ensifer adhaerens | |
22.5 | - |
1,5-Anhydro-D-fructose | mutant K94G in the presence of NADPH | Ensifer adhaerens | |
39 | - |
1,5-Anhydro-D-fructose | mutant A13G/S10G in the presence of NADH | Ensifer adhaerens | |
49 | - |
1,5-Anhydro-D-fructose | mutant D176A in the presence of NADPH | Ensifer adhaerens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ensifer adhaerens | - |
- |
- |
Ensifer adhaerens S-30.7.5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Ensifer adhaerens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADH | - |
Ensifer adhaerens | 1,5-anhydro-D-mannitol + NAD+ | - |
? | |
1,5-anhydro-D-fructose + NADH | - |
Ensifer adhaerens S-30.7.5 | 1,5-anhydro-D-mannitol + NAD+ | - |
? | |
1,5-anhydro-D-fructose + NADPH | - |
Ensifer adhaerens | 1,5-anhydro-D-mannitol + NADP+ | - |
ir | |
1,5-anhydro-D-fructose + NADPH | - |
Ensifer adhaerens S-30.7.5 | 1,5-anhydro-D-mannitol + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
1,4-AF-reductase | - |
Ensifer adhaerens |
AFR | - |
Ensifer adhaerens |
NADP(H)-dependent 1,5-anhydro-D-fructose reductase | - |
Ensifer adhaerens |
NADPH-dependent anhydrofructose reductase | - |
Ensifer adhaerens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
1,5-Anhydro-D-fructose | mutant D176A in the presence of NADPH | Ensifer adhaerens | |
3.7 | - |
1,5-Anhydro-D-fructose | mutant H108A in the presence of NADPH | Ensifer adhaerens | |
4.2 | - |
1,5-Anhydro-D-fructose | mutant K94G in the presence of NADPH | Ensifer adhaerens | |
5.5 | - |
1,5-Anhydro-D-fructose | mutant A13G/S10G in the presence of NADH | Ensifer adhaerens | |
6.3 | - |
1,5-Anhydro-D-fructose | mutant A13G/S33D in the presence of NADPH | Ensifer adhaerens | |
12.4 | - |
1,5-Anhydro-D-fructose | mutant A13G in the presence of NADH | Ensifer adhaerens | |
13.5 | - |
1,5-Anhydro-D-fructose | mutant S33D in the presence of NADH | Ensifer adhaerens | |
119 | - |
1,5-Anhydro-D-fructose | mutant S10G in the presence of NADPH | Ensifer adhaerens | |
145 | - |
1,5-Anhydro-D-fructose | recombinant wild type enzyme in the presence of NADPH | Ensifer adhaerens | |
156 | - |
1,5-Anhydro-D-fructose | mutant G206I in the presence of NADPH | Ensifer adhaerens | |
216 | - |
1,5-Anhydro-D-fructose | native wild type enzyme in the presence of NADPH | Ensifer adhaerens | |
369 | - |
1,5-Anhydro-D-fructose | mutant A13G/S10Gin the presence of NADPH | Ensifer adhaerens | |
405 | - |
1,5-Anhydro-D-fructose | mutant A13G in the presence of NADPH | Ensifer adhaerens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Ensifer adhaerens |