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Literature summary for 1.1.1.25 extracted from

  • Peek, J.; Shi, T.; Christendat, D.
    Identification of novel polyphenolic inhibitors of shikimate dehydrogenase (AroE) (2014), J. Biomol. Screen., 19, 1090-1098.
    View publication on PubMed
Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus Arabidopsis thaliana
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 2O7S Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
2,2-bisepigallocatechin gallate about 50% inhibition at 0.0025 mM Pseudomonas putida
aurintricarboxylic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
baicalein about 25% inhibition at 0.0025 mM Pseudomonas putida
cardiolipin lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
dianthrol about 50% inhibition at 0.0025 mM Pseudomonas putida
diethylenetriamine pentaacetic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
ebselen lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
ellagic acid about 50% inhibition at 0.0025 mM Pseudomonas putida
epicatechin gallate inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana Arabidopsis thaliana
epicatechin gallate over 75% inhibition at 0.0025 mM Pseudomonas putida
epigallocatechin gallate inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana Arabidopsis thaliana
epigallocatechin gallate over 75% inhibition at 0.0025 mM Pseudomonas putida
epigallocatechin-3,5-digallate about 50% inhibition at 0.0025 mM Pseudomonas putida
epitheaflavin monogallate about 50% inhibition at 0.0025 mM Pseudomonas putida
hydroquinone lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
merbromin lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
additional information screening for polyphenolc enzyme inhibitors, overview Arabidopsis thaliana
additional information screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin Pseudomonas putida
nordihydroguaiaretic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
purpurogallin about 50% inhibition at 0.0025 mM Pseudomonas putida
pyridoxine lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
pyrogallin about 50% inhibition at 0.0025 mM Pseudomonas putida
quercetin about 25% inhibition at 0.0025 mM Pseudomonas putida
taxifolin about 25% inhibition at 0.0025 mM Pseudomonas putida
theaflavanin about 25% inhibition at 0.0025 mM Pseudomonas putida
theaflavin monogallate about 50% inhibition at 0.0025 mM Pseudomonas putida
theaflavin-3,3-digallate about 25% inhibition at 0.0025 mM Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydroshikimate + NADPH Arabidopsis thaliana
-
 shikimate + NADP+
-
 r
shikimate + NADP+ Pseudomonas putida
-
 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+ Pseudomonas putida KT 2240
-
 3-dehydroshikimate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SQT8
-
-
Pseudomonas putida Q88RQ5
-
-
Pseudomonas putida KT 2240 Q88RQ5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis Arabidopsis thaliana
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroshikimate + NADPH
-
 Arabidopsis thaliana shikimate + NADP+
-
 r
additional information The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate Arabidopsis thaliana ?
-
 ?
shikimate + NADP+
-
 Pseudomonas putida 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+
-
 Pseudomonas putida KT 2240 3-dehydroshikimate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
AroE
-
 Arabidopsis thaliana
AroE
-
 Pseudomonas putida
bifunctional dehydroquinate dehydratase-shikimate dehydrogenase
-
 Arabidopsis thaliana
DHQ-SDH
-
 Arabidopsis thaliana
SDH
-
 Arabidopsis thaliana
SDH
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana
25
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Arabidopsis thaliana
8.8
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Arabidopsis thaliana
NADP+
-
 Pseudomonas putida
NADPH
-
 Arabidopsis thaliana
NADPH
-
 Pseudomonas putida

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
 pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme Arabidopsis thaliana epicatechin gallate
0.0021
-
 pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme Arabidopsis thaliana epigallocatechin gallate
0.003
-
 pH 8.8, 25°C, recombinant enzyme Pseudomonas putida epigallocatechin gallate
0.0037
-
 pH 8.8, 25°C, recombinant enzyme Pseudomonas putida epicatechin gallate