Cloned (Comment) | Organism |
---|---|
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus | Arabidopsis thaliana |
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis, PDB ID 2O7S | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2-bisepigallocatechin gallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
aurintricarboxylic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
baicalein | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
cardiolipin | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
dianthrol | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
diethylenetriamine pentaacetic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
ebselen | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
ellagic acid | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
epicatechin gallate | inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana | Arabidopsis thaliana | |
epicatechin gallate | over 75% inhibition at 0.0025 mM | Pseudomonas putida | |
epigallocatechin gallate | inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana | Arabidopsis thaliana | |
epigallocatechin gallate | over 75% inhibition at 0.0025 mM | Pseudomonas putida | |
epigallocatechin-3,5-digallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
epitheaflavin monogallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
hydroquinone | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
merbromin | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
additional information | screening for polyphenolc enzyme inhibitors, overview | Arabidopsis thaliana | |
additional information | screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin | Pseudomonas putida | |
nordihydroguaiaretic acid | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
purpurogallin | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
pyridoxine | lower inhibitry potency, about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
pyrogallin | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
quercetin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
taxifolin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
theaflavanin | about 25% inhibition at 0.0025 mM | Pseudomonas putida | |
theaflavin monogallate | about 50% inhibition at 0.0025 mM | Pseudomonas putida | |
theaflavin-3,3-digallate | about 25% inhibition at 0.0025 mM | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroshikimate + NADPH | Arabidopsis thaliana | - |
shikimate + NADP+ | - |
r | |
shikimate + NADP+ | Pseudomonas putida | - |
3-dehydroshikimate + NADPH + H+ | - |
r | |
shikimate + NADP+ | Pseudomonas putida KT 2240 | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SQT8 | - |
- |
Pseudomonas putida | Q88RQ5 | - |
- |
Pseudomonas putida KT 2240 | Q88RQ5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis | Arabidopsis thaliana |
recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroshikimate + NADPH | - |
Arabidopsis thaliana | shikimate + NADP+ | - |
r | |
additional information | The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate | Arabidopsis thaliana | ? | - |
? | |
shikimate + NADP+ | - |
Pseudomonas putida | 3-dehydroshikimate + NADPH + H+ | - |
r | |
shikimate + NADP+ | - |
Pseudomonas putida KT 2240 | 3-dehydroshikimate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Arabidopsis thaliana |
AroE | - |
Pseudomonas putida |
bifunctional dehydroquinate dehydratase-shikimate dehydrogenase | - |
Arabidopsis thaliana |
DHQ-SDH | - |
Arabidopsis thaliana |
SDH | - |
Arabidopsis thaliana |
SDH | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Arabidopsis thaliana |
8.8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana | |
NADP+ | - |
Pseudomonas putida | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Pseudomonas putida |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme | Arabidopsis thaliana | epicatechin gallate | |
0.0021 | - |
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme | Arabidopsis thaliana | epigallocatechin gallate | |
0.003 | - |
pH 8.8, 25°C, recombinant enzyme | Pseudomonas putida | epigallocatechin gallate | |
0.0037 | - |
pH 8.8, 25°C, recombinant enzyme | Pseudomonas putida | epicatechin gallate |