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Literature summary for 1.1.1.25 extracted from

  • Schoepe, J.; Niefind, K.; Schomburg, D.
    1.6 A structure of an NAD(+)-dependent quinate dehydrogenase from Corynebacterium glutamicum (2008), Acta Crystallogr. Sect. D, 64, 803-809.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
diffraction to 1.6 A Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.2
-
quinate pH 9.0, 30°C Corynebacterium glutamicum
46.6
-
shikimate pH 10.0, 30°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q9X5C9 quinate/shikimate dehydrogenase
-

Reaction

Reaction Comment Organism Reaction ID
shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+ active-site base is K92. After the binding of quinate and NAD+, the oxygen of the C3-OH of quinate forms a hydrogen bond to the side chain of the conserved T88 and K92 functions as the active-site base to remove the proton on the C3-OH. Simultaneously,a hydride is transferred from C3 of quinate to NAD+ Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinate + NAD+ in contrast to shikimate, quinate may form a hydrogen bond to the NAD+, and the hydroxyl group of a active-site threonine hydrogen bonds to quinate more effectively than shikimate resulting in a lower Michaelis constant and higher catalytic efficiency for quinate Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
?
shikimate + NAD+
-
Corynebacterium glutamicum 3-dehydroshikimate + NADH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
61.9
-
quinate pH 9.0, 30°C Corynebacterium glutamicum
85.2
-
shikimate pH 10.0, 30°C Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred over NADP+ Corynebacterium glutamicum
NADP+ turnover number decreases by more than 300fold when NADP+ is used instead of NAD+, regardless of substrate shikimate or quinate Corynebacterium glutamicum