Literature summary for 1.1.1.25 extracted from

Singh, S.; Korolev, S.; Koroleva, O.; Zarembinski, T.; Collart, F.; Joachimiak, A.; Christendat, D.
Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae (2005), J. Biol. Chem., 280, 17101-17108.

Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
phylogenetic analysis of the three paralogues YdiB, AroE, and HI0607, DNA and amino acid sequence determination and analysis of paralogue HI0607, expression of wild-type paralogue HI0607 in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in strain B834(DE3)	Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled paralogue HI0607, hanging drop vapour diffusion method, 0.001 ml protein solution containing 20 mg/ml protein mixed with equal volume of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 2.0 M NaCl, and 10% v/v PEG 400, X-ray diffraction structure determination and analysis at 1.75 A resolution	Haemophilus influenzae

Protein Variants

Protein Variants	Comment	Organism
D103X	site-directed mutagenesis of paralogue HI0607, inactive mutant	Haemophilus influenzae
K67H	site-directed mutagenesis of paralogue HI0607, inactive mutant	Haemophilus influenzae

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition of paralogue HI0607 by EDTA	Haemophilus influenzae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	paralogue HI0607 is not affected by 5 mM of Zn2+, Ca2+, Mg2+, or Mn2+	Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
quinate + NADP+	Haemophilus influenzae	reversible activity of YdiB, no activity with paralogue HI0607	3-dehydroquinate + NADPH + H+	-	r
shikimate + NADP+	Haemophilus influenzae	reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity	3-dehydroshikimate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	-	three paralogues: YdiB, AroE, and the shikimate dehydrogenase-like HI0607	-

Reaction

Reaction	Comment	Organism	Reaction ID
shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+	conserved residues Asp103 and Lys67 are important for catalysis in all three paralogues, kinetic mechanism of paralogue HI0607 and substrate binding site	Haemophilus influenzae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
quinate + NADP+	reversible activity of YdiB, no activity with paralogue HI0607	Haemophilus influenzae	3-dehydroquinate + NADPH + H+	-	r
shikimate + NADP+	reversible activity of YdiB and AroE, paralogue HI0607 catalyzes the oxidative reaction with low activity	Haemophilus influenzae	3-dehydroshikimate + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	recombinant paralogue HI0607, SDS-PAGE and gel filtration	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
AroE	-	Haemophilus influenzae
HI0607	-	Haemophilus influenzae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Haemophilus influenzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	9.8	paralogue HI0607	Haemophilus influenzae
8.5	-	assay at	Haemophilus influenzae

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	pH profile, paralogue HI0607	Haemophilus influenzae

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	cofactor binding site of paralogue HI0607	Haemophilus influenzae
NADPH	-	Haemophilus influenzae

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Release 2023.1 (January 2023)