Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Nonidet P-40 | 120-190% activity of AroE at 0.1-0.5% | Mycobacterium tuberculosis | |
Triton X-100 | 120-190% activity at 0.1-0.5% | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene aroE, expression of His-tagged AroE in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
the purified recombinant enzyme is stable to freezing | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | nearly complete inactivation of AroE at 0.02% | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic profile of the recombinant AroE, overview | Mycobacterium tuberculosis | |
0.0295 | - |
NADP+ | pH 9.0, 25°C | Mycobacterium tuberculosis | |
0.063 | - |
shikimate | pH 9.0, 25°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Mycobacterium tuberculosis | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene aroE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged ARoE from Escherichia coli strain BL21(DE3) by nickel affinity chromatograohy | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
826 | - |
purified recombinant AroE | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-70°C, purified recombinant enzyme, stable for several months | Mycobacterium tuberculosis |
4°C, purified recombinant enzyme, stable for 2 weeks | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | - |
Mycobacterium tuberculosis | 3-dehydroshikimate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis of ARoE, percentages for alpha-helix, beta-sheet, beta-turn, and random coil are 29.2%, 9.3%, 32.7%, and 28.8%, respectively, contains the highly conserved motif G-X-(N/S)-V-(T/S)-X-PX-K | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Mycobacterium tuberculosis |
SD | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
87 | - |
purified recombinant AroE, half-life: 2 h | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
399 | - |
NADP+ | pH 9.0, 25°C | Mycobacterium tuberculosis | |
399 | - |
shikimate | pH 9.0, 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 12 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |