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Literature summary for 1.1.1.25 extracted from
- Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production (2006), Biosci. Biotechnol. Biochem., 70, 2786-2789.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, native PAGE | Gluconobacter oxydans |
| 30000 | - |
2 * 30000, native PAGE | Gluconobacter oxydans |
| 60000 | - |
gel filtration and native PAGE | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| shikimate + NADP+ | Gluconobacter oxydans | - |
3-dehydroshikimate + NADPH + H+ | - |
r |  |
| shikimate + NADP+ | Gluconobacter oxydans IFO 3244 | - |
3-dehydroshikimate + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans IFO 3244 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:quinate-grown cell | - |
Gluconobacter oxydans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| shikimate + NADP+ | - |
Gluconobacter oxydans | 3-dehydroshikimate + NADPH + H+ | - |
r | |
| shikimate + NADP+ | - |
Gluconobacter oxydans IFO 3244 | 3-dehydroshikimate + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, native PAGE | Gluconobacter oxydans |
| monomer | 1 * 30000, native PAGE | Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-dependent shikimate dehydrogenase | - |
Gluconobacter oxydans |
| SKDH | - |
Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reduction of dehydroshikimate | Gluconobacter oxydans |
| 10 | - |
oxidation of shikimate | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Gluconobacter oxydans | |
| NADPH | - |
Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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