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Literature summary for 1.1.1.25 extracted from

  • Lourenco, E.J.; Silva, G.M.L.; Neves, V.A.
    Purification and properties of shikimate dehydrogenase from cucumber (Cucumis sativus L.). (1991), J. Agric. Food Chem., 39, 458-462.
    View publication on PubMed

General Stability

General Stability Organism
stable in crude extract Cucumis sativus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 500 nM Cucumis sativus
HgCl2 complete inhibition at concentration 0.05 mM Cucumis sativus
p-chloromercuribenzoate biphasic inhibition: first rapid inhibition leading to loss of 70% activity, then within 5 min loss of the remaining 30% activity, inhibition can be partially hindered by thiols and chloride Cucumis sativus
protocatechuic acid
-
Cucumis sativus
Zn2+ 0.005 mM Cucumis sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADP+
-
Cucumis sativus
0.05
-
shikimic acid NADP+ Cucumis sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration, 2 different isoenzymes with molecular weight about 45000 Da Cucumis sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
shikimate + NADP+ Cucumis sativus
-
3-dehydroshikimate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cucumis sativus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
crude extract Cucumis sativus
25.4
-
purified enzyme Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
shikimate + NADP+
-
Cucumis sativus 3-dehydroshikimate + NADPH + H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Cucumis sativus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55
-
Cucumis sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
complete inactivation after 10 min, at 50-55°C biphasic, inactivation can be minimized with bound substrate to about 60% Cucumis sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Cucumis sativus