Literature summary for 1.1.1.248 extracted from

Kempe, K.; Higashi, Y.; Frick, S.; Sabarna, K.; Kutchan, T.M.
RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes (2009), Phytochemistry, 70, 579-589.

Search for more literature for 1.1.1.248

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL	Papaver somniferum

Cloned (Comment)

Organism

cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL

Papaver somniferum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident	Papaver somniferum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Papaver somniferum	5829	-

Organism

Organism	UniProt	Comment	Textmining
Papaver somniferum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged SalR purified with a cobalt affinity resin	Papaver somniferum

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Papaver somniferum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
salutaridine + NADPH + H+	interaction between SalR and SalAT in morphine biosynthesis	Papaver somniferum	(7S)-salutaridinol + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
SalR	-	Papaver somniferum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Papaver somniferum