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Literature summary for 1.1.1.234 extracted from

  • Fischer, T.C.; Halbwirth, H.; Meisel, B.; Stich, K.; Forkmann, G.
    Molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from Malus domestica and Pyrus communis cultivars and the consequences for flavonoid metabolism (2003), Arch. Biochem. Biophys., 412, 223-230.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast strain InvSc1 Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-eriodictyol + NADPH Pyrus communis reaction provides precursors for the synthesis of 3-deoxyanthocyanidins luteoforol + NADP+ reaction provides precursors for the synthesis of 3-deoxyanthocyanidins ?
(2S)-eriodictyol + NADPH Malus domestica reaction provides precursors for the synthesis of 3-deoxyanthocyanidins luteoforol + NADP+ reaction provides precursors for the synthesis of 3-deoxyanthocyanidins ?
(2S)-flavanone + NADPH Pyrus communis reaction provides precursors for the synthesis of 3-deoxyanthocyanidins (2S)-flavan-4-ol + NADP+
-
?
(2S)-flavanone + NADPH Malus domestica reaction provides precursors for the synthesis of 3-deoxyanthocyanidins (2S)-flavan-4-ol + NADP+
-
?
(2S)-naringenin + NADPH + H+ Pyrus communis
-
apiforol + NADP+
-
?
(2S)-naringenin + NADPH + H+ Malus domestica
-
apiforol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Malus domestica Q9XES5 strains M9 and Weirouge
-
Pyrus communis
-
strains Pyrodwarf, Conference and Abbe Fetel
-

Reaction

Reaction Comment Organism Reaction ID
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH + H+ enzyme acts as flavone 4-reductase as well as dihydroflavonol 4-reductase Pyrus communis
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH + H+ enzyme acts as flavone 4-reductase as well as dihydroflavonol 4-reductase Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Malus domestica
-
leaf low activity Pyrus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-eriodictyol + NADPH
-
Pyrus communis luteoforol + NADP+
-
?
(2S)-eriodictyol + NADPH
-
Malus domestica luteoforol + NADP+
-
?
(2S)-eriodictyol + NADPH reaction provides precursors for the synthesis of 3-deoxyanthocyanidins Pyrus communis luteoforol + NADP+ reaction provides precursors for the synthesis of 3-deoxyanthocyanidins ?
(2S)-eriodictyol + NADPH reaction provides precursors for the synthesis of 3-deoxyanthocyanidins Malus domestica luteoforol + NADP+ reaction provides precursors for the synthesis of 3-deoxyanthocyanidins ?
(2S)-flavanone + NADPH
-
Pyrus communis (2S)-flavan-4-ol + NADP+
-
?
(2S)-flavanone + NADPH
-
Malus domestica (2S)-flavan-4-ol + NADP+
-
?
(2S)-flavanone + NADPH reaction provides precursors for the synthesis of 3-deoxyanthocyanidins Pyrus communis (2S)-flavan-4-ol + NADP+
-
?
(2S)-flavanone + NADPH reaction provides precursors for the synthesis of 3-deoxyanthocyanidins Malus domestica (2S)-flavan-4-ol + NADP+
-
?
(2S)-naringenin + NADPH + H+
-
Pyrus communis apiforol + NADP+
-
?
(2S)-naringenin + NADPH + H+
-
Malus domestica apiforol + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
native enzyme Pyrus communis
25
-
native enzyme Malus domestica
40
-
recombinant enzyme from yeast Pyrus communis
40
-
recombinant enzyme from yeast Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pyrus communis
6
-
-
Malus domestica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pyrus communis
NADPH
-
Malus domestica