General Stability | Organism |
---|---|
degassed buffers stabilize | Apium graveolens |
DTT stabilizes | Apium graveolens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 47% reduction by 100 mM | Apium graveolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.8 | - |
D-mannose 6-phosphate | - |
Apium graveolens | |
45 | - |
D-mannitol 1-phosphate | - |
Apium graveolens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Apium graveolens | 5737 | - |
cytoplasm | - |
Ligustrum vulgare | 5737 | - |
protoplast | - |
Apium graveolens | - |
- |
protoplast | - |
Ligustrum vulgare | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Apium graveolens |
53000 | - |
gel filtration with Superose 12 | Apium graveolens |
58000 | - |
gel filtration with Sephacryl S-200 | Apium graveolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apium graveolens | - |
celery | - |
Apium graveolens | - |
cv. Giant Pascal | - |
Ligustrum vulgare | - |
privet | - |
Oxidation Stability | Organism |
---|---|
DTT stabilizes | Apium graveolens |
Purification (Comment) | Organism |
---|---|
- |
Apium graveolens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Apium graveolens | - |
shoot | - |
Ligustrum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.053 | - |
- |
Apium graveolens |
3.76 | - |
purified from leaves | Apium graveolens |
Storage Stability | Organism |
---|---|
-21°C, 50% activity retained after 1 month | Apium graveolens |
4°C, lyophilized active fractions stable for several months | Apium graveolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannose 6-phosphate + NADPH | - |
Apium graveolens | D-mannitol 1-phosphate + NADP+ | - |
r | |
D-mannose 6-phosphate + NADPH | no activity with D-fructose 6-phosphate, D-glucose 6-phosphate, D-fructose 1-phosphate, D-mannose 1-phosphate, D-mannose | Ligustrum vulgare | D-mannitol 1-phosphate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Apium graveolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
D-mannose 6-phosphate | Apium graveolens |
8.5 | - |
D-mannitol 1-phosphate | Apium graveolens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | inactivated at pH below 6 | Apium graveolens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Apium graveolens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate | Apium graveolens | |
NADP+ | NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate | Ligustrum vulgare | |
NADPH | NADH cannot replace NADPH in reducing mannose 6-phosphate | Apium graveolens | |
NADPH | NADH cannot replace NADPH in reducing mannose 6-phosphate | Ligustrum vulgare |