Application | Comment | Organism |
---|---|---|
molecular biology | the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems | Granulibacter bethesdensis |
synthesis | the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems | Granulibacter bethesdensis |
Cloned (Comment) | Organism |
---|---|
recombinant enzyme overexpression | Granulibacter bethesdensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | strong inhibition | Granulibacter bethesdensis | |
UDP-xylose | allosteric feedback inhibitor, reduces significantly the activity of the enzyme | Granulibacter bethesdensis | |
Urea | strong inhibition | Granulibacter bethesdensis | |
Zn2+ | strong inhibition | Granulibacter bethesdensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 2 NAD+ + H2O | Granulibacter bethesdensis | - |
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Granulibacter bethesdensis | - |
originally isolated from the lymph nodes of patients with chronic granulomatous disease (CGD) | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, simple purification method | Granulibacter bethesdensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Granulibacter bethesdensis | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? | |
UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Granulibacter bethesdensis | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates | ? |
Synonyms | Comment | Organism |
---|---|---|
GbUGD | - |
Granulibacter bethesdensis |
UDP-glucose dehydrogenase | - |
Granulibacter bethesdensis |
UDPglucose dehydrogenase | - |
Granulibacter bethesdensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Granulibacter bethesdensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Granulibacter bethesdensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Granulibacter bethesdensis | |
NAD+ | specific for | Granulibacter bethesdensis |