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Literature summary for 1.1.1.22 extracted from

  • Ge, X.; Penney, L.C.; van de Rijn, I.; Tanner, M.E.
    Active site residues and mechanism of UDP-glucose dehydrogenase (2004), Eur. J. Biochem., 271, 14-22.
    View publication on PubMed
Search for more literature for 1.1.1.22

Protein Variants

Protein Variants Comment Organism
C260A no oxidation of UDP-glucose to glucuronic acid, but capable of both reducing the aldehyde intermediate and oxidizing the hydrated form of the aldehyde intermediate, protein is expressed in inclusion bodies Streptococcus pyogenes
E141Q kcat-value 10fold lower than wild-type Streptococcus pyogenes
E145Q kcat-value 10fold lower than wild-type Streptococcus pyogenes
T118A 160fold reduction of kcat value Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 UDP-glucose wild-type Streptococcus pyogenes
0.058
-
 aldehyde intermediate
-
 Streptococcus pyogenes
0.059
-
 UDP-glucose mutant T118A Streptococcus pyogenes
0.06
-
 UDP-glucose mutant E141Q Streptococcus pyogenes
0.065
-
 NAD+ wild-type Streptococcus pyogenes
0.125
-
 UDP-glucose mutant E145Q Streptococcus pyogenes
0.135
-
 NAD+ mutant E141Q Streptococcus pyogenes
0.187
-
 NAD+ mutant E145Q Streptococcus pyogenes
0.4
-
 NAD+ mutant T118A Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + 2 NAD+ + H2O = UDP-alpha-D-glucuronate + 2 NADH + 2 H+ mechanism, ordered water molecule H-bonded to T118 serves as catalytic base in hydride transfer Streptococcus pyogenes
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
 Streptococcus pyogenes UDP-glucuronate + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 aldehyde intermediate
-
 Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Streptococcus pyogenes