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Literature summary for 1.1.1.219 extracted from

  • Bulut, D.; Duangdee, N.; Groeger, H.; Berkessel, A.; Hummel, W.
    Screening, molecular cloning, and biochemical characterization of an alcohol dehydrogenase from Pichia pastoris useful for the kinetic resolution of a racemic beta-hydroxy-beta-trifluoromethyl ketone (2016), ChemBioChem, 17, 1349-1358.
    View publication on PubMed
Search for more literature for 1.1.1.219

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Komagataella phaffii

Protein Variants

Protein Variants Comment Organism
additional information fusion of ADH to the N-terminal His-tag causes a strong improvement of about 95% in activity Komagataella phaffii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0161
-
 NADPH pH 7.0, 25°C Komagataella phaffii
1.15
-
 (4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one pH 7.0, 25°C Komagataella phaffii
5.2
-
 benzaldehyde pH 7.0, 25°C Komagataella phaffii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
-
 Komagataella phaffii
41400
-
-
 Komagataella phaffii

Organism

Organism UniProt Comment Textmining
Komagataella phaffii C4R4L0
-
-
Komagataella phaffii GS115 C4R4L0
-
-

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity in 100 days Komagataella phaffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-hex-2-enal + NADPH + H+ 97% of the activity with benzaldehyde Komagataella phaffii (2E)-hex-2-en-1-ol + NADP+
-
 ?
(2E)-hex-2-enal + NADPH + H+ 97% of the activity with benzaldehyde Komagataella phaffii GS115 (2E)-hex-2-en-1-ol + NADP+
-
 ?
(2S)-hexan-2-ol + NADP+
-
 Komagataella phaffii hexan-2-one + NADPH + H+
-
 r
(2S)-hexan-2-ol + NADP+
-
 Komagataella phaffii GS115 hexan-2-one + NADPH + H+
-
 r
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one + NADPH + H+ enzyme specifically reduces the S-enantiomer Komagataella phaffii (2S)-1,1,1-trifluoro-2-phenylpentane-2,4-diol + NADP+
-
 ?
2-methylpentanal + NADPH + H+ 249% of the activity with benzaldehyde Komagataella phaffii 2-methyl-pentan-1-ol + NADP+
-
 ?
benzaldehyde + NADPH + H+
-
 Komagataella phaffii benzyl alcohol + NADP+
-
 ?
benzaldehyde + NADPH + H+
-
 Komagataella phaffii GS115 benzyl alcohol + NADP+
-
 ?
butanal + NADPH + H+ 176% of the activity with benzaldehyde Komagataella phaffii butan-1-ol + NADP+
-
 ?
butanal + NADPH + H+ 176% of the activity with benzaldehyde Komagataella phaffii GS115 butan-1-ol + NADP+
-
 ?
heptan-2-one + NADPH + H+
-
 Komagataella phaffii (2S)-heptan-2-ol + NADP+ 99% conversion, 99% enantiomeric excess r
hexan-2-one + NADPH + H+
-
 Komagataella phaffii (2S)-hexan-2-ol + NADP+ 99% conversion, 99% enantiomeric excess r
hexanal + NADPH + H+ 74% of the activity with benzaldehyde Komagataella phaffii hexan-1-ol + NADP+
-
 ?
additional information linear and branched aliphatic aldehydes are good substrates Komagataella phaffii ?
-
 ?
additional information linear and branched aliphatic aldehydes are good substrates Komagataella phaffii GS115 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE, x * 41400, calculated Komagataella phaffii

Synonyms

Synonyms Comment Organism
PAS_chr3_0449
-
 Komagataella phaffii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Komagataella phaffii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, rapid inactivation Komagataella phaffii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.5
-
 (4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one pH 7.0, 25°C Komagataella phaffii
54.4
-
 benzaldehyde pH 7.0, 25°C Komagataella phaffii
58.4
-
 NADPH pH 7.0, 25°C Komagataella phaffii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Komagataella phaffii

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
 Komagataella phaffii