Cloned (Comment) | Organism |
---|---|
gene AKR1C26, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hexestrol | - |
Oryctolagus cuniculus | |
hinokitiol | - |
Oryctolagus cuniculus | |
icarisid II | - |
Oryctolagus cuniculus | |
ikarisoside A | - |
Oryctolagus cuniculus | |
kaempferol | - |
Oryctolagus cuniculus | |
noranhydroicaritin | - |
Oryctolagus cuniculus | |
Phenolphthalein | - |
Oryctolagus cuniculus | |
quercetin | - |
Oryctolagus cuniculus | |
quercitrin | - |
Oryctolagus cuniculus | |
zearalenone | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Oryctolagus cuniculus | |
0.02 | - |
NAD+ | pH 7.4, 25°C | Oryctolagus cuniculus | |
0.027 | - |
trans-naphthalene dihydrodiol | pH 7.4, 25°C | Oryctolagus cuniculus | |
0.033 | - |
NADH | pH 7.4, 25°C | Oryctolagus cuniculus | |
0.52 | - |
morphine | pH 7.4, 25°C | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
morphine + NAD+ | Oryctolagus cuniculus | - |
morphinone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | J7M9D0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | high expression level of AKR1C26 | Oryctolagus cuniculus | - |
kidney | high expression level of AKR1C26 | Oryctolagus cuniculus | - |
liver | high expression level of AKR1C26 | Oryctolagus cuniculus | - |
additional information | ubiquitous expression in rabbit tissues | Oryctolagus cuniculus | - |
stomach | high expression level of AKR1C26 | Oryctolagus cuniculus | - |
testis | high expression level of AKR1C26 | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows NAD+-dependent dehydrogenase activity towards other nonsteroidal alicyclic alcohols and 3alpha/17beta-hydroxy-C18/C19/C21-steroids, detailed overview | Oryctolagus cuniculus | ? | - |
? | |
morphine + NAD+ | - |
Oryctolagus cuniculus | morphinone + NADH + H+ | - |
? | |
trans-naphthalene dihydrodiol + NAD+ | - |
Oryctolagus cuniculus | ? + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M6DH | - |
Oryctolagus cuniculus |
More | cf. EC 1.1.1.239 | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NAD+ | pH 7.4, 25°C | Oryctolagus cuniculus | |
0.067 | - |
trans-naphthalene dihydrodiol | pH 7.4, 25°C | Oryctolagus cuniculus | |
0.49 | - |
morphine | pH 7.4, 25°C | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the rabbit enzyme shows high coenzyme preference for NAD+ over NADP+. Also in the reverse reaction, the enzymes reduces several alpha-dicarbonyl compounds including S-camphorquinone using NADH as the coenzyme, exhibiting low Km values of 0.004-0.006 mM. The reductase activity of the enzyme determined with NADPH as the coenzyme is less than 40% of their NADH-linked activity | Oryctolagus cuniculus | |
NAD+ | - |
Oryctolagus cuniculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0064 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | noranhydroicaritin | |
0.007 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | Phenolphthalein | |
0.013 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | ikarisoside A | |
0.02 | - |
above, pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | kaempferol | |
0.026 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | quercetin | |
0.037 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | hinokitiol | |
0.04 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | quercitrin | |
0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | zearalenone | |
0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | icarisid II | |
0.073 | - |
pH 7.4, 25°C, recombinant enzyme | Oryctolagus cuniculus | Hexestrol |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme M6DH is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Oryctolagus cuniculus |
physiological function | mammalian morphine 6-dehydrogenase converts morphine into a reactive electrophile, morphinone. M6DH is involved in the toxic action of morphine including the development of tolerance to this drug | Oryctolagus cuniculus |