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Literature summary for 1.1.1.216 extracted from

  • Inoue, H.; Tsuji, H.; Uritani, I.
    Characteriziation and activity change of farnesol dehydrogenase in black rot fungus-infected sweet potato (1984), Agric. Biol. Chem., 48, 733-738.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CuCl2
-
Ipomoea batatas
EDTA
-
Ipomoea batatas
Monoiodoacetamide
-
Ipomoea batatas
additional information inhibitory effect of thiol reagents indicates that the SH-group of the enzyme participates in its activity Ipomoea batatas
N-ethylmaleimide
-
Ipomoea batatas
ZnCl2
-
Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
trans,trans-farnesol
-
Ipomoea batatas
0.729
-
geraniol
-
Ipomoea batatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Ipomoea batatas
90000
-
gel filtation Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas
-
sweet potato, enzyme is formed in response to infection by the black rot fungus Ceratocystis fimbriata
-

Purification (Commentary)

Purification (Comment) Organism
-
Ipomoea batatas

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Ipomoea batatas
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis,trans-farnesol + NADP+
-
Ipomoea batatas cis,trans-farnesal + NADPH
-
?
citronellol + NADP+
-
Ipomoea batatas ?
-
?
decanol + NADP+
-
Ipomoea batatas decanal + NADPH
-
?
geraniol + NADP+
-
Ipomoea batatas ?
-
?
nerol + NADP+
-
Ipomoea batatas ?
-
?
trans,trans-farnesol + NADP+ preferred substrate Ipomoea batatas trans,trans-farnesal + NADPH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Ipomoea batatas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ipomoea batatas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 reduction of trans,trans-farnesal Ipomoea batatas
9.5
-
oxidation of trans,trans-farnesol Ipomoea batatas

pH Range

pH Minimum pH Maximum Comment Organism
6 9 reduction of trans,trans-farnesal: 60% of maximal activity at pH 6.5 and at pH 8 Ipomoea batatas
7 10.5 oxidation of trans,trans-farnesol: 10% of maximal activity at pH 7.0, 30% of maximal activity at pH 10.2 Ipomoea batatas

Cofactor

Cofactor Comment Organism Structure
NADP+ NAD+ cannot replace NADP+ Ipomoea batatas