Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | expression and activity of cj0414 and cj0415 are higher at 42°C than at 37°C | Campylobacter jejuni |
Application | Comment | Organism |
---|---|---|
medicine | ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts | Campylobacter jejuni |
Cloned (Comment) | Organism |
---|---|
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176 | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Campylobacter jejuni | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26900 | - |
sequence analysis, cj0414 | Campylobacter jejuni |
63700 | - |
sequence analysis, cj0415 | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Campylobacter jejuni 81-176 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization | Campylobacter jejuni | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization | Campylobacter jejuni 81-176 | 2-dehydro-D-gluconate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GADH | - |
Campylobacter jejuni |
gluconate dehydrogenase | - |
Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Campylobacter jejuni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Campylobacter jejuni | cj0414, isoelectric focusing | - |
5.5 |
Campylobacter jejuni | cj0415, isoelectric focusing | - |
8.8 |