Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression of wild-type and expression of start codon mutated enzyme in Escherichia coli strain DH5alpha | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is located on the periplasmic side of the cytoplasmic membrane | Gluconobacter oxydans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | Gluconobacter oxydans | - |
2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | Gluconobacter oxydans 621H | - |
2-dehydro-D-gluconate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FTU6 | gene GOX0417 | - |
Gluconobacter oxydans 621H | Q5FTU6 | gene GOX0417 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | - |
Gluconobacter oxydans | 2-dehydro-D-gluconate + NADPH | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter oxydans 621H | 2-dehydro-D-gluconate + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2KGA reductase | - |
Gluconobacter oxydans |
2KGR | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | bound by the middle subunit | Gluconobacter oxydans | |
FAD | enzyme bound to the large subunit | Gluconobacter oxydans | |
NADP+ | - |
Gluconobacter oxydans |