Application | Comment | Organism |
---|---|---|
synthesis | enzyme is useful for production of chiral alcohols | Candida parapsilosis |
Cloned (Comment) | Organism |
---|---|
isozyme CPR-C2, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme | Candida parapsilosis |
isozymes CPR-C1, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme | Candida parapsilosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-pantolactone | substrate inhibition of the recombinant isozyme expressed in Escherichia coli at a concentration above 10 mg/ml | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, recombinant isozyme CPR-C2, SDS-PAGE | Candida parapsilosis |
38000 | - |
x * 38000, recombinant isozyme CPR-C1, SDS-PAGE | Candida parapsilosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantolactone + NADP+ | Candida parapsilosis | - |
2-dehydropantolactone + NADPH | - |
? | |
(R)-pantolactone + NADP+ | Candida parapsilosis IFO 0708 | - |
2-dehydropantolactone + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | Q76L36 | isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2 | - |
Candida parapsilosis | Q76L37 | isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2 | - |
Candida parapsilosis IFO 0708 | Q76L36 | isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2 | - |
Candida parapsilosis IFO 0708 | Q76L37 | isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.03 | - |
recombinant isozyme CPR-C2 in cell extract of expressing Escherichia coli cells in absence of IPTG | Candida parapsilosis |
19.3 | - |
recombinant isozyme CPR-C1 in cell extract of expressing Escherichia coli cells in absence of IPTG | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantolactone + NADP+ | - |
Candida parapsilosis | 2-dehydropantolactone + NADPH | - |
? | |
(R)-pantolactone + NADP+ | i.e. ketopantoyl lactone | Candida parapsilosis | 2-dehydropantolactone + NADPH | - |
? | |
(R)-pantolactone + NADP+ | i.e. ketopantoyl lactone | Candida parapsilosis IFO 0708 | 2-dehydropantolactone + NADPH | - |
? | |
(R)-pantolactone + NADP+ | - |
Candida parapsilosis IFO 0708 | 2-dehydropantolactone + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, recombinant isozyme CPR-C2, SDS-PAGE | Candida parapsilosis |
? | x * 38000, recombinant isozyme CPR-C1, SDS-PAGE | Candida parapsilosis |
Synonyms | Comment | Organism |
---|---|---|
CPR-C1 | - |
Candida parapsilosis |
CPR-C2 | - |
Candida parapsilosis |
ketopantoyl lactone reductase | - |
Candida parapsilosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Candida parapsilosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
about | Candida parapsilosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Candida parapsilosis |