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Literature summary for 1.1.1.214 extracted from

  • Kataoka, M.; Delacruz-Hidalgo, A.R.G.; Akond, M.A.; Sakuradani, E.; Kita, K.; Shimizu, S.
    Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis. Investigation of hydroxamic acids as laccase-mediators for pulp bleaching (2004), Appl. Microbiol. Biotechnol., 64, 359-366.
    View publication on PubMed

Application

Application Comment Organism
synthesis enzyme is useful for production of chiral alcohols Candida parapsilosis

Cloned(Commentary)

Cloned (Comment) Organism
isozyme CPR-C2, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme Candida parapsilosis
isozymes CPR-C1, DNA and amino acid sequence determination and analysis, overexpression of the isozyme in Escherichia coli strain BL21(DE3), addition of IPTG highly decreases the expression rate of the isozyme Candida parapsilosis

Inhibitors

Inhibitors Comment Organism Structure
(R)-pantolactone substrate inhibition of the recombinant isozyme expressed in Escherichia coli at a concentration above 10 mg/ml Candida parapsilosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, recombinant isozyme CPR-C2, SDS-PAGE Candida parapsilosis
38000
-
x * 38000, recombinant isozyme CPR-C1, SDS-PAGE Candida parapsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-pantolactone + NADP+ Candida parapsilosis
-
2-dehydropantolactone + NADPH
-
?
(R)-pantolactone + NADP+ Candida parapsilosis IFO 0708
-
2-dehydropantolactone + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Candida parapsilosis Q76L36 isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2
-
Candida parapsilosis Q76L37 isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2
-
Candida parapsilosis IFO 0708 Q76L36 isozyme CPR-C2; 2 isozymes CPR-C1 and CPR-C2
-
Candida parapsilosis IFO 0708 Q76L37 isozyme CPR-C1; 2 isozymes CPR-C1 and CPR-C2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.03
-
recombinant isozyme CPR-C2 in cell extract of expressing Escherichia coli cells in absence of IPTG Candida parapsilosis
19.3
-
recombinant isozyme CPR-C1 in cell extract of expressing Escherichia coli cells in absence of IPTG Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantolactone + NADP+
-
Candida parapsilosis 2-dehydropantolactone + NADPH
-
?
(R)-pantolactone + NADP+ i.e. ketopantoyl lactone Candida parapsilosis 2-dehydropantolactone + NADPH
-
?
(R)-pantolactone + NADP+ i.e. ketopantoyl lactone Candida parapsilosis IFO 0708 2-dehydropantolactone + NADPH
-
?
(R)-pantolactone + NADP+
-
Candida parapsilosis IFO 0708 2-dehydropantolactone + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 36000, recombinant isozyme CPR-C2, SDS-PAGE Candida parapsilosis
? x * 38000, recombinant isozyme CPR-C1, SDS-PAGE Candida parapsilosis

Synonyms

Synonyms Comment Organism
CPR-C1
-
Candida parapsilosis
CPR-C2
-
Candida parapsilosis
ketopantoyl lactone reductase
-
Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Candida parapsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
about Candida parapsilosis

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
Candida parapsilosis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Candida parapsilosis