Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
keto-omega-methylpantoyl lactone + NADPH | the only compound other than ketopantoyl lactone which is a substrate, 73% relative activity to ketopantoyl lactone with form A enzyme, 56% relative activity to ketopantoyl lactone with form B enzyme | Escherichia coli | methylpantoyl lactone + NADP+ | - |
? | |
ketopantoyl lactone + NADPH | - |
Escherichia coli | pantoyl lactone + NADP+ | - |
? | |
additional information | both forms of the enzyme are B-specific, the enzyme exhibits opposite stereospecificity from that observed with the Saccharomyces cerevisiae enzyme, which is A-specific, less than 5% relative activity to ketopantoyl lactone with the following 2-keto-gamma-lactones: 2-keto-4-hydroxy-3-methylbutyric acid-gamma-lactone, 2-keto-4-hydroxybutyric acid-gamma-lactone | Escherichia coli | ? | - |
? | |
additional information | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | Escherichia coli | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity observed with NADH | Escherichia coli | |
NADPH | - |
Escherichia coli |