Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in ternary/binary complex with inhibitor nitazoxanide and/or NADP+, or with NADP+ and glyceraldehyde, for enzyme-NADP+-nitazoxanide complex, enzyme-NADP+ crystals are soaked in a saturated solution of nitazoxanide containing 50% N,N-dimethylformamide, 50 mM ammonium citrate, pH 5.0, and 12.5% PEG 6000, for enzyme-NADP+-D-glyceraldehyde complex, enzyme-NADP+ crystals are soaked in a solution containing 20% pyridine, 0.3 M D,L-glyceraldehyde, 50 mM ammonium citrate, pH 5.0, and 12.5% m/v PEG 6000, X-ray diffraction structure determination and analysis, modeling | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epalrestat | full inhibition | Rattus norvegicus | |
nitazoxanide | partial inhibition | Rattus norvegicus | |
tizoxanide | partial inhibition | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | the enzyme catalyzes the reduction of a wide range of substances, including prostaglandin H2, phospholipid aldehydes, lipid peroxidation products, environmental pollutants and their glutathione conjugates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the reduction of a wide range of substances, including prostaglandin H2, phospholipid aldehydes, lipid peroxidation products, environmental pollutants and their glutathione conjugates | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
aldose reductase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Rattus norvegicus | |
0.00935 | - |
tizoxanide | pH and temperature not specified in the publication | Rattus norvegicus | |
0.01355 | - |
nitazoxanide | pH and temperature not specified in the publication | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00011 | - |
pH and temperature not specified in the publication | Rattus norvegicus | epalrestat | |
0.00535 | - |
pH and temperature not specified in the publication | Rattus norvegicus | tizoxanide | |
0.00822 | - |
pH and temperature not specified in the publication | Rattus norvegicus | nitazoxanide |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is the first and rate-limiting enzyme in the polyol pathway where it catalyzes the reduction of glucose to sorbitol | Rattus norvegicus |