Cloned (Comment) | Organism |
---|---|
overexpression | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H110A | mutant enzyme | Homo sapiens |
H110Q | mutant enzyme | Homo sapiens |
K77M | mutant enzyme | Homo sapiens |
Y48F | mutant enzyme | Homo sapiens |
Y48H | changing substrate selectivity | Homo sapiens |
Y48S | mutant enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | inhibition is pH-dependent | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
DL-glyceraldehyde | - |
Homo sapiens | |
7.4 | - |
D-xylose | - |
Homo sapiens | |
12 | - |
DL-glyceraldehyde | mutant H110Q | Homo sapiens | |
64 | - |
DL-glyceraldehyde | mutant Y48S | Homo sapiens | |
71 | - |
DL-glyceraldehyde | mutant Y48H | Homo sapiens | |
84 | - |
DL-glyceraldehyde | mutant H110A | Homo sapiens | |
111 | - |
L-Xylose | - |
Homo sapiens | |
360 | - |
L-Xylose | mutant H110A | Homo sapiens | |
506 | - |
D-Lyxose | mutant H110Q | Homo sapiens | |
861 | - |
D-xylose | mutant H110Q | Homo sapiens | |
1190 | - |
L-Xylose | mutant H110Q | Homo sapiens | |
1340 | - |
D-xylose | mutant H110A | Homo sapiens | |
2630 | - |
D-Lyxose | - |
Homo sapiens | |
2740 | - |
D-Lyxose | mutant H110A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alditol + NAD(P)+ = aldose + NAD(P)H + H+ | reaction mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzaldehyde + NAD(P)H | - |
Homo sapiens | 4-nitrobenzyl alcohol + NAD(P)+ | - |
? | |
D-lyxose + NADPH | - |
Homo sapiens | arabitol + NADP+ | - |
? | |
DL-glyceraldehyde + NAD(P)H | - |
Homo sapiens | glycerol + NAD(P)+ | - |
r | |
L-xylose + NAD(P)H | - |
Homo sapiens | L-xylitol + NAD(P)+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
DL-glyceraldehyde | turnover number in mol per seconds for several mutants | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH-specific, pro-4R hydrogen is transferred from the nicotinamide ring of the coenzyme to the substrate | Homo sapiens |