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Literature summary for 1.1.1.21 extracted from

  • Bohren, K.M.; Grimshaw, C.E.; Lai, C.J.; Harrison, D.H.; Ringe, D.; Petsko, G.A.; Gabbay, K.H.
    Tyrosine-48 is the proton donor and histidine-110 directs substrate stereochemical selectivity in the reduction reaction of human aldose reductase: enzyme kinetics and crystal structure of the Y48H mutant enzyme (1994), Biochemistry, 33, 2021-2032.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
H110A mutant enzyme Homo sapiens
H110Q mutant enzyme Homo sapiens
K77M mutant enzyme Homo sapiens
Y48F mutant enzyme Homo sapiens
Y48H changing substrate selectivity Homo sapiens
Y48S mutant enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
citrate inhibition is pH-dependent Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
DL-glyceraldehyde
-
Homo sapiens
7.4
-
D-xylose
-
Homo sapiens
12
-
DL-glyceraldehyde mutant H110Q Homo sapiens
64
-
DL-glyceraldehyde mutant Y48S Homo sapiens
71
-
DL-glyceraldehyde mutant Y48H Homo sapiens
84
-
DL-glyceraldehyde mutant H110A Homo sapiens
111
-
L-Xylose
-
Homo sapiens
360
-
L-Xylose mutant H110A Homo sapiens
506
-
D-Lyxose mutant H110Q Homo sapiens
861
-
D-xylose mutant H110Q Homo sapiens
1190
-
L-Xylose mutant H110Q Homo sapiens
1340
-
D-xylose mutant H110A Homo sapiens
2630
-
D-Lyxose
-
Homo sapiens
2740
-
D-Lyxose mutant H110A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
alditol + NAD(P)+ = aldose + NAD(P)H + H+ reaction mechanism Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenzaldehyde + NAD(P)H
-
Homo sapiens 4-nitrobenzyl alcohol + NAD(P)+
-
?
D-lyxose + NADPH
-
Homo sapiens arabitol + NADP+
-
?
DL-glyceraldehyde + NAD(P)H
-
Homo sapiens glycerol + NAD(P)+
-
r
L-xylose + NAD(P)H
-
Homo sapiens L-xylitol + NAD(P)+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
DL-glyceraldehyde turnover number in mol per seconds for several mutants Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH-specific, pro-4R hydrogen is transferred from the nicotinamide ring of the coenzyme to the substrate Homo sapiens