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Literature summary for 1.1.1.206 extracted from

  • Chen, W.; Cheng, X.; Zhou, Z.; Liu, J.; Wang, H.
    Molecular cloning and characterization of a tropinone reductase from Dendrobium nobile Lindl (2013), Mol. Biol. Rep., 40, 1145-1154.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene DnTR1, sequence comparisons and unrooted phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), realtime PCR enzyme expression analysis Dendrobium nobile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Dendrobium nobile
0.167
-
3-quinuclidinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
5.61
-
tropinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
8.71
-
4-methylcyclohexanone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
-
Dendrobium nobile
60000
-
gel filtration, recombinant enzyme Dendrobium nobile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Dendrobium nobile
-
tropine + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Dendrobium nobile H9BQR7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration Dendrobium nobile

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Dendrobium nobile
-
additional information DnTR1 may be ubiquitously expressed in all tissues, expression profile of DnTR1 Dendrobium nobile
-
root
-
Dendrobium nobile
-
stem
-
Dendrobium nobile
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-quinuclidinone + NADPH + H+
-
Dendrobium nobile 1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
r
4-methylcyclohexanone + NADPH + H+
-
Dendrobium nobile 4-methylcyclohexanol + NADP+
-
r
tropinone + NADPH + H+
-
Dendrobium nobile tropine + NADP+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 28000, recombinant enzyme, SDS-PAGE Dendrobium nobile
More three-dimensional structure modelling of DnTR1, overview Dendrobium nobile

Synonyms

Synonyms Comment Organism
DnTR1
-
Dendrobium nobile

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Dendrobium nobile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Dendrobium nobile

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Dendrobium nobile
NADPH
-
Dendrobium nobile

Expression

Organism Comment Expression
Dendrobium nobile salicylic acid and sodium nitroprusside treatments do not affect the enzyme expression additional information
Dendrobium nobile the expression of DnTR1 mRNA is enhanced 9.5times by treatment of methyl jasmonate after 24 h, expression profile of DnTR1 up

General Information

General Information Comment Organism
evolution the enzyme belongs to the short chain dehydrogenase/reductase (SDR) superfamily that is composed of a group of NAD(P)H-dependent oxidoreductases that typically consist of 250-350 amino acids. Tropinone reductases (TRs) are a group of the SDR superfamily, that use the NADPH as coenzyme to reduce tropinone DnTR1 also contains the sequence pattern G-X3-G-X-G in the cofactor binding motif and active site motif (S-N-K) Dendrobium nobile
metabolism tropinone reductases form a branch point in the pathway leading to tropine (TRI) and to pseudotropine (TRII) during the tropane alkaloid biosynthesis, overview. Enzyme TRI is involved in the formation of tropine, the preproduct of hyoscyamine, whereas enzyme TRII, EC 1.1.1.236, is responsible for the generation of pseudotropine Dendrobium nobile
additional information three-dimensional structure modelling of DnTR1, overview. DnTR1 also contains the sequence pattern G-X3-G-X-G in the cofactor binding motif and active site motif (S-N-K). The positive and negative charges on the binding pockets surface of DsTRI are due to His112. The three catalytic residues are Ser158, Tyr171, and Lys175 in DsTRI Dendrobium nobile
physiological function DnTR1 regulation may be involved in a jasmonate-dependent pathway Dendrobium nobile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00094
-
tropinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
0.0032
-
4-methylcyclohexanone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
22.18
-
3-quinuclidinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile