Literature summary for 1.1.1.206 extracted from

Kai, G.; Li, L.; Jiang, Y.; Yan, X.; Zhang, Y.; Lu, X.; Liao, P.; Chen, J.
Molecular cloning, characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloids production in AaTRI-transformed hairy roots (2009), Biotechnol. Appl. Biochem., 54, :177-186.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	methyl jasmonate can enhance expression of TRI, with weak effect for TRI. TRI is induced weakly in all tissues	Anisodus acutangulus

Cloned(Commentary)

Cloned (Comment)	Organism
TRI-transformed hairy root lines. Full-length fragment of TRI cDNA insert constructed into the pBI121 expression cassette in place of GUS gene. The vector pCAMBIA1304+ contains the TRI gene under control of CaMV35S promoter. Disarmed Agrobacterium tumefaciens strain C58C1 harboring both Agrobacterium rhizogenes Ri plasmid pRiA4 and pCAMBIA1304+, containing a single TRI gene, used for plant transformation	Anisodus acutangulus

Cloned (Comment)

Organism

TRI-transformed hairy root lines. Full-length fragment of TRI cDNA insert constructed into the pBI121 expression cassette in place of GUS gene. The vector pCAMBIA1304+ contains the TRI gene under control of CaMV35S promoter. Disarmed Agrobacterium tumefaciens strain C58C1 harboring both Agrobacterium rhizogenes Ri plasmid pRiA4 and pCAMBIA1304+, containing a single TRI gene, used for plant transformation

Anisodus acutangulus

Inhibitors

Inhibitors	Comment	Organism	Structure
methyl jasmonate	inhibits expression of TRI in hairy roots	Anisodus acutangulus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29460	-	sequence analysis	Anisodus acutangulus

Organism

Organism	UniProt	Comment	Textmining
Anisodus acutangulus	B2L2W8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	transcript level of TRI is low	Anisodus acutangulus	-
root	transcript level of TRI is low	Anisodus acutangulus	-
stem	transcript level of TRI is low	Anisodus acutangulus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	significant increase of tropane alkaloids after TRI single gene transformation in Anisodus acutangulus	Anisodus acutangulus	?	-	?

Synonyms

Synonyms	Comment	Organism
TRI	-	Anisodus acutangulus
tropinone reductase	-	Anisodus acutangulus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Anisodus acutangulus	sequence analysis	-	6.45