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Literature summary for 1.1.1.205 extracted from

  • Makowska-Grzyska, M.; Kim, Y.; Maltseva, N.; Osipiuk, J.; Gu, M.; Zhang, M.; Mandapati, K.; Gollapalli, D.R.; Gorla, S.K.; Hedstrom, L.; Joachimiak, A.
    A novel cofactor-binding mode in bacterial IMP dehydrogenases explains inhibitor selectivity (2015), J. Biol. Chem., 290, 5893-5911.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.205

Application

Application Comment Organism
medicine MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents Clostridium perfringens
medicine MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents Vibrio cholerae serotype O1
medicine MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents Campylobacter jejuni
medicine MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long and short enzyme CBS-deletion mutants, BaIMPDHDELTAL and BaIMPDHDELTAS, in Escherichia coli Bacillus anthracis
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long enzyme CBS-deletion mutant, ClpIMPDHDELTAL, in Escherichia coli Clostridium perfringens
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long enzyme CBS-deletion mutant, VcIMPDHDELTAL, in Escherichia coli Vibrio cholerae serotype O1
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged short enzyme CBS-deletion mutant, CjIMPDHDELTAS, in Escherichia coli Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant long enzyme mutant ClpIMPDHDELTAL in complex with IMP and with inhibitor 1 from 5% tacsimate, pH 7.0, 0.1 M HEPES, pH 7.0, 10% PEG MME 5000, 16°C, or with inhibitor 2 from 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG 10000, 16°C, X-ray diffraction structure determination and analysis at resolutions of 2.95 and 2.85 A, respectively Clostridium perfringens
purified recombinant long enzyme mutant VcIMPDHDELTAL in complex with IMP and NAD+ from 0.77 M sodium/potassium phosphate, 0.15 M Tris-HCl, pH 8.0, 6% MPD, 16°C, followed by soaking with 200 mM NAD+ solution for 15 min at 20°C, or enzyme mutant VcIMPDHDELTAL in complex with XMP and NADH from 1.03 M sodium/potassium phosphate, pH 5.0, 0.15 M sodium malate, 3% PEG 300, 16°C, followed by soaking with 200 mM NAD+ solution for 5 days at 16°C, X-ray diffraction structure determination and analysis at resolutions of 2.38 and 1.66 A, respectively Vibrio cholerae serotype O1
purified recombinant short enzyme mutant BaIMPDHDELTAS in apoform from 0.2 M sodium chloride, 0.1 M sodium cacodylate, pH 6.5, and 2 M ammonium sulfate, 16°C, and purified recombinant long enzyme mutant BaIMPDHDELTAL in complex with IMP and with inhibitors 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide, ((alpha-methyl-)N-2-naphthalenyl-2-(2-pyridinyl)-1H-benzimidazole-)1-acetamide, 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide, 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide, N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea, and 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide in several conformations and under different conditions resulting in 6 different crystal structures, X-ray diffraction structure determination and analysis at resolutions of 1.93-2.85 A, respectively, overview Bacillus anthracis
purified recombinant short enzyme mutant CjIMPDHDELTAS in complex with IMP and with inhibitor 2 from 1.6 M ammonium sulfate, 0.1 M MES, pH 6.5, 10% dioxane, 16 °C, or with inhibitor 4 from 0.2 M lithium sulfate, 0.1 M CAPS, pH 10.5, 1.2 M sodium/0.8 M potassium phosphate, 16 °C, X-ray diffraction structure determination and analysis at resolutions of 2.44 and 2.54 A, respectively Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
additional information construction of a long CBS domain deletion mutant variant, ClpIMPDHDELTAL. Deletion of residues Gln89-Arg215 Clostridium perfringens
additional information construction of a long CBS domain deletion mutant variant, VcIMPDHDELTAL. Deletion of residues Phe91-Arg219 Vibrio cholerae serotype O1
additional information construction of a short and a long CBS domain deletion mutant variant, BaIMPDHDELTAS and BaIMPDHDELTAL. Deletion of residues Val95-Thr200 and Glu92-Arg220, respectively Bacillus anthracis
additional information construction of a short CBS domain deletion mutant variant, CjIMPDHDELTAS. Deletion of residues Val92-Thr195 Campylobacter jejuni

Inhibitors

Inhibitors Comment Organism Structure
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
-
 Bacillus anthracis
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
-
 Campylobacter jejuni
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
-
 Clostridium perfringens
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
-
 Vibrio cholerae serotype O1
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
-
 Bacillus anthracis
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
-
 Campylobacter jejuni
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
-
 Clostridium perfringens
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
-
 Vibrio cholerae serotype O1
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
-
 Bacillus anthracis
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
-
 Campylobacter jejuni
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
-
 Clostridium perfringens
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
-
 Vibrio cholerae serotype O1
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
-
 Bacillus anthracis
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
-
 Campylobacter jejuni
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
-
 Clostridium perfringens
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
-
 Vibrio cholerae serotype O1
additional information inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variants, overview Bacillus anthracis
additional information inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variant, overview Campylobacter jejuni
additional information inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variant, overview Clostridium perfringens
additional information inhibitor synthesis, overview Vibrio cholerae serotype O1
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
-
 Bacillus anthracis
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
-
 Campylobacter jejuni
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
-
 Clostridium perfringens
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
-
 Vibrio cholerae serotype O1
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
-
 Bacillus anthracis
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
-
 Campylobacter jejuni
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
-
 Clostridium perfringens
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
-
 Vibrio cholerae serotype O1
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Bacillus anthracis
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Campylobacter jejuni
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Clostridium perfringens
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Vibrio cholerae serotype O1
NAD+ substrate inhibition Bacillus anthracis
NAD+ substrate inhibition Campylobacter jejuni
NAD+ substrate inhibition Clostridium perfringens
NAD+ substrate inhibition Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni
0.049
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens
0.055
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni
0.061
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis
0.064
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis
0.08
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Vibrio cholerae serotype O1
0.087
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL Vibrio cholerae serotype O1
0.1
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens
0.15
-
 IMP pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis
0.22
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni
0.37
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens
0.46
-
 NAD+ pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis
0.51
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens
0.52
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni
0.55
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis
0.56
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis
1.1
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL Vibrio cholerae serotype O1
1.2
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51943
-
-
 Vibrio cholerae serotype O1
52093
-
-
 Clostridium perfringens
52151
-
-
 Campylobacter jejuni
52374
-
-
 Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IMP + NAD+ + H2O Clostridium perfringens
-
 XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O Vibrio cholerae serotype O1
-
 XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O Campylobacter jejuni
-
 XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O Bacillus anthracis
-
 XMP + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-
Campylobacter jejuni
-
-
-
Clostridium perfringens
-
-
-
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged long and short enzyme CBS-deletion mutants, BaIMPDHDELTAL and BaIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis Bacillus anthracis
recombinant His-tagged long enzyme CBS-deletion mutant, ClpIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis Clostridium perfringens
recombinant His-tagged long enzyme CBS-deletion mutant, VcIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis Vibrio cholerae serotype O1
recombinant His-tagged short enzyme CBS-deletion mutant, CjIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis Campylobacter jejuni

Reaction

Reaction Comment Organism Reaction ID
IMP + NAD+ + H2O = XMP + NADH + H+ the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP Clostridium perfringens
a
IMP + NAD+ + H2O = XMP + NADH + H+ the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP Vibrio cholerae serotype O1
a
IMP + NAD+ + H2O = XMP + NADH + H+ the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP Campylobacter jejuni
a
IMP + NAD+ + H2O = XMP + NADH + H+ the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP Bacillus anthracis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IMP + NAD+ + H2O
-
 Clostridium perfringens XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O
-
 Vibrio cholerae serotype O1 XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O
-
 Campylobacter jejuni XMP + NADH + H+
-
 ?
IMP + NAD+ + H2O
-
 Bacillus anthracis XMP + NADH + H+
-
 ?
additional information IMP- and XMP-enzyme binding structure analysis, overview Vibrio cholerae serotype O1 ?
-
 ?
additional information IMP-enzyme binding structure analysis, overview Clostridium perfringens ?
-
 ?
additional information IMP-enzyme binding structure analysis, overview Campylobacter jejuni ?
-
 ?
additional information IMP-enzyme binding structure analysis, overview Bacillus anthracis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37929, long enzyme truncation CBS-mutant VcIMPDHDELTAL, sequence calculation Vibrio cholerae serotype O1
tetramer 4 * 38123, long enzyme truncation CBS-mutant ClpIMPDHDELTAL, sequence calculation Clostridium perfringens
tetramer 4 * 40435, long enzyme truncation CBS-mutant BaIMPDHDELTAL, sequence calculation, 4 * 37920, short enzyme truncation CBS-mutant BaIMPDHDELTAL, sequence calculation Bacillus anthracis
tetramer 4 * 40722, short enzyme truncation CBS-mutant CjIMPDHDELTAS, sequence calculation Campylobacter jejuni

Synonyms

Synonyms Comment Organism
IMP dehydrogenase
-
 Clostridium perfringens
IMP dehydrogenase
-
 Vibrio cholerae serotype O1
IMP dehydrogenase
-
 Campylobacter jejuni
IMP dehydrogenase
-
 Bacillus anthracis
IMPDH
-
 Clostridium perfringens
IMPDH
-
 Vibrio cholerae serotype O1
IMPDH
-
 Campylobacter jejuni
IMPDH
-
 Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Clostridium perfringens
25
-
 assay at Vibrio cholerae serotype O1
25
-
 assay at Campylobacter jejuni
25
-
 assay at Bacillus anthracis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens
1.9
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni
2.1
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Vibrio cholerae serotype O1
2.2
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni
2.9
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens
4.5
-
 IMP pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis
5.2
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL Vibrio cholerae serotype O1
5.3
-
 IMP pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis
6.1
-
 IMP pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Clostridium perfringens
8
-
 assay at Vibrio cholerae serotype O1
8
-
 assay at Campylobacter jejuni
8
-
 assay at Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme binding structure analysis, overview Clostridium perfringens
NAD+ enzyme binding structure analysis, overview Vibrio cholerae serotype O1
NAD+ enzyme binding structure analysis, overview Campylobacter jejuni
NAD+ enzyme binding structure analysis, overview Bacillus anthracis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.8
-
 NAD+ pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis
3.9
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis
5.3
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis
6.4
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni
9
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens
13
-
 NAD+ pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens
13
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni
13
-
 NAD+ pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL Vibrio cholerae serotype O1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 the inhibitors all exhibit inhibitory potencies with IC50 values of over 0.005 mM with wild-type and truncation mutant Vibrio cholerae serotype O1 additional information
0.000006
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.00001
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000013
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000015
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.000019
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000019
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.00002
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000022
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000023
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000024
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000027
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000028
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000029
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000034
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000036
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.000038
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000039
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.00004
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000042
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.000043
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.000044
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.000046
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000047
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000051
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.000054
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.000063
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.000065
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.000066
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea
0.000067
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea
0.000067
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.000072
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.000074
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.000092
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide
0.000095
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide
0.0001
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.000107
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS Campylobacter jejuni 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.000137
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS Bacillus anthracis 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.00014
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.000169
-
 pH 8.0, 25°C, recombinant wild-type enzyme Campylobacter jejuni 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.0002
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant3 CjIMPDHDELTAS Campylobacter jejuni 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.000364
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide
0.00045
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.00057
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
0.00067
-
 pH 8.0, 25°C, recombinant wild-type enzyme Bacillus anthracis 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.0007
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL Bacillus anthracis 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.0008
-
 pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant3 BaIMPDHDELTAS Bacillus anthracis 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.0017
-
 pH 8.0, 25°C, recombinant wild-type enzyme Clostridium perfringens 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide
0.0034
-
 pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL Clostridium perfringens 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide

General Information

General Information Comment Organism
malfunction the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation Clostridium perfringens
malfunction the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation Vibrio cholerae serotype O1
malfunction the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation Campylobacter jejuni
malfunction the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation Bacillus anthracis
metabolism the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis Clostridium perfringens
metabolism the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis Vibrio cholerae serotype O1
metabolism the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis Campylobacter jejuni
metabolism the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis Bacillus anthracis
additional information the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* Clostridium perfringens
additional information the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* Vibrio cholerae serotype O1
additional information the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* Campylobacter jejuni
additional information the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* Bacillus anthracis