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Literature summary for 1.1.1.205 extracted from

  • Xu, D.; Cobb, G.; Spellicy, C.J.; Bowne, S.J.; Daiger, S.P.; Hedstrom, L.
    Retinal isoforms of inosine 5-monophosphate dehydrogenase type 1 are poor nucleic acid binding proteins (2008), Arch. Biochem. Biophys., 472, 100-104.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression as fusion protein with green fluorescence protein, in HEK-293 cell, HeLa cell and COS cell, and expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D226N mutant of isoform IMPDH1(546), and mutant of isoform IMPDH1(595), both are localized to cytosol and exhibit kinetic parameters identical to wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information retinal isoforms do not bind significant fractions of a random pool of oligonucleotides Homo sapiens
NAD+ at high concentration, inhibition of canonical isoform. No inhibition of retinal-specific isoforms Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
inosine 5'-phosphate isoform IMPDH1(514), pH 8.0, 25°C Homo sapiens
0.01
-
inosine 5'-phosphate mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens
0.011
-
inosine 5'-phosphate isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens
0.011
-
inosine 5'-phosphate mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.014
-
NAD+ isoform IMPDH1(514), pH 8.0, 25°C Homo sapiens
0.015
-
inosine 5'-phosphate isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.016
-
NAD+ mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens
0.022
-
NAD+ isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.024
-
NAD+ mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.026
-
NAD+ isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
type 1 enzyme, retinal-specific isoforms IMPDH1(546) and IMPDH1(595) and canonical isoform IMPDH1(514)
-

Source Tissue

Source Tissue Comment Organism Textmining
retina retinal-specific isoforms IMPDH1(546) and IMPDH1(595) Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Homo sapiens xanthosine 5'-phosphate + NADH
-
?

Subunits

Subunits Comment Organism
More the C-terminal extension unique to the retinal isoforms of IMPDH blocks the nucleic acid binding site Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
inosine 5'-phosphate isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens
0.22
-
inosine 5'-phosphate isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.22
-
inosine 5'-phosphate mutant D226N of isoform IMPDH1(595), pH 8.0, 25°C Homo sapiens
0.24
-
inosine 5'-phosphate mutant D226N of isoform IMPDH1(546), pH 8.0, 25°C Homo sapiens
0.31
-
inosine 5'-phosphate isoform IMPDH1(514), pH 8.0, 25°C Homo sapiens