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Literature summary for 1.1.1.205 extracted from
- The purification of inosine 5-monophosphate dehydrogenase from Escherichia coli by affinity chromatography on immobilized Procion dyes (1980), Anal. Biochem., 104, 23-28.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GMP | competitive with respect to IMP | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inosine 5'-phosphate + NAD+ + H2O | Escherichia coli | - |
xanthosine 5'-phosphate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
wild type and guaA mutants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Escherichia coli |
| native enzyme about 14fold by affinity chromatography on different immobilized dyes, elution by salt gradient, over 90% purity of the enzyme, method development and optimization, overview | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 58000, identical subunits | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMP:NAD+ oxidoreductase | - |
Escherichia coli |
| inosine 5 -monophosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.6 | assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
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