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Literature summary for 1.1.1.203 extracted from
- Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli (2009), Appl. Environ. Microbiol., 75, 589-595.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas syringae pv. tomato |
| Udh subcloned into vector pTrc99A to produce pT1053 and transformed into Escherichia coli BL21 Star (DE3) to construct an Escherichia coli strain carrying INO1, MIOX, and udh | Pseudomonas syringae pv. tomato |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae pv. tomato | Q888H1 | - |
- |
| Pseudomonas syringae pv. tomato | Q888H1 | pv. tomato DC3000 | - |
| Pseudomonas syringae pv. tomato DC3000 | Q888H1 | - |
- |
| Pseudomonas syringae pv. tomato DC3000 | Q888H1 | pv. tomato DC3000 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
value about, culture condition 1-3 days, 30°C | Pseudomonas syringae pv. tomato |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucuronate + NAD+ + H2O | inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid | Pseudomonas syringae pv. tomato | D-glucaric acid + NADH + H+ | - |
? |  |
| D-glucuronate + NAD+ + H2O | inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid | Pseudomonas syringae pv. tomato DC3000 | D-glucaric acid + NADH + H+ | - |
? | |
| D-glucuronic acid + NAD+ + H2O | assay at pH 8.0 | Pseudomonas syringae pv. tomato | D-glucaric acid + NADH + H+ | - |
? | |
| D-glucuronic acid + NAD+ + H2O | assay at pH 8.0 | Pseudomonas syringae pv. tomato DC3000 | D-glucaric acid + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Udh | - |
Pseudomonas syringae pv. tomato |
| uronate dehydrogenase | - |
Pseudomonas syringae pv. tomato |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas syringae pv. tomato |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas syringae pv. tomato | |
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Release 2023.1 (January 2023)
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