General Stability | Organism |
---|---|
DTT and dithioerythritol have protective function against thermal destruction | Pseudomonas syringae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | 15% inhibition at 1 mM | Pseudomonas syringae | |
N-ethylmaleimide | inhibition at 1 mM, reversal by an excess of L-cysteine | Pseudomonas syringae | |
NADH | competitive inhibition, Ki: 0.06 mM | Pseudomonas syringae | |
p-chloromercuribenzoic acid | 60% inhibition at 1 mM, reversal by an excess of L-cysteine | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
D-galacturonate | - |
Pseudomonas syringae | |
0.08 | - |
NAD+ | - |
Pseudomonas syringae | |
0.37 | - |
D-glucuronate | - |
Pseudomonas syringae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
2 * 30500, SDS-PAGE, subunits alone inactive | Pseudomonas syringae |
59000 | - |
gel filtration, ultracentrifugation | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucuronate + NAD+ | Pseudomonas syringae | weak reversible reaction, 3% of the activity compared to the forward reaction | D-glucaric acid 3,6-lactone + NADH + H+ | - |
r | |
D-glucuronate + NAD+ + H2O | Pseudomonas syringae | - |
D-glucaric acid + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Pseudomonas syringae |
Renatured (Comment) | Organism |
---|---|
treatment with 8 M urea and 24 h dialysis: 10% of initial activity | Pseudomonas syringae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas syringae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
81 | - |
- |
Pseudomonas syringae |
Storage Stability | Organism |
---|---|
-20°C, 50 mM sodium diphosphate-HCl, pH 8.0, 0.1% bovine serum albumin, no loss of activity | Pseudomonas syringae |
4°C, 50 mM sodium diphosphate-HCl, pH 8.0, 30% glycerol, 5% loss of activity within 1 month | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NAD+ + H2O | - |
Pseudomonas syringae | D-galactarate + NADH + H+ | - |
? | |
D-glucuronate + NAD+ | weak reversible reaction, 3% of the activity compared to the forward reaction | Pseudomonas syringae | D-glucaric acid 3,6-lactone + NADH + H+ | - |
r | |
D-glucuronate + NAD+ + H2O | - |
Pseudomonas syringae | D-glucaric acid + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30500, SDS-PAGE, subunits alone inactive | Pseudomonas syringae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas syringae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
15 | stable for 24 h | Pseudomonas syringae |
25 | - |
15% loss of activity within 2 h | Pseudomonas syringae |
37 | - |
90% loss of activity within 60 min | Pseudomonas syringae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.7 | - |
D-galacturonate | - |
Pseudomonas syringae | |
80 | - |
D-glucuronate | - |
Pseudomonas syringae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas syringae |