General Stability | Organism |
---|---|
-20°C, Mn2+-DTT-HEPES buffer, stable for 3 days, 82% loss of activity after 3 weeks | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Klebsiella pneumoniae | |
2,2'-dipyridyl | reactivation by Mn2+ or Fe2+ | Klebsiella pneumoniae | |
8-hydroxyquinoline | - |
Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
NAD+ | - |
Klebsiella pneumoniae | |
18 | - |
Propane-1,3-diol | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * or 8 * 45000, SDS-PAGE | Klebsiella pneumoniae |
328000 | - |
gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,3-diol + NAD+ | Klebsiella pneumoniae | - |
3-hydroxypropanal + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Oxidation Stability | Organism |
---|---|
inactivated by oxidation during aerobic metabolism | Klebsiella pneumoniae |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,3-diol + NAD+ | - |
Klebsiella pneumoniae | 3-hydroxypropanal + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer or octamer | 6 * or 8 * 45000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADP+ has no coenzyme activity | Klebsiella pneumoniae | |
NAD+ | - |
Klebsiella pneumoniae | |
NADH | - |
Klebsiella pneumoniae |