Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7beta-hydroxycholesterol + NADP+ | Oryctolagus cuniculus | - |
7-oxocholesterol + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.276 | - |
purified enzyme | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-cholesten-3beta,7beta-diol + NADP+ | i.e. 7beta-hydroxycholesterol | Oryctolagus cuniculus | 5-cholesten-3beta-ol-7-one + NADPH | - |
? | |
7beta-hydroxycholesterol + NADP+ | - |
Oryctolagus cuniculus | 7-oxocholesterol + NADPH | - |
? | |
corticosterone + NADP+ | oxidation at C-11 | Oryctolagus cuniculus | 21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH | - |
? | |
cortisone + NADP+ | oxidation at C-11 | Oryctolagus cuniculus | ? + NADPH | - |
? | |
additional information | enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
7beta-HSD | - |
Oryctolagus cuniculus |
7beta-hydroxysteroid dehydrogenase | - |
Oryctolagus cuniculus |
More | enzyme might belong to the rabbit type I 11beta-HSD enzyme family | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
potassium buffer | Oryctolagus cuniculus |
11 | - |
glycine-NaOH buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | beta-form is preferred | Oryctolagus cuniculus |