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Literature summary for 1.1.1.200 extracted from
- Characterization and partial purification of aldose-6-phosphate reductase (alditol-6-phosphate:NADP 1-oxidoreductase) from apple leaves (1981), Plant Physiol., 67, 139-142.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | complete inhibition at 0.1 mM | Malus domestica |  |
| cysteine | 47% inhibition of oxidation and 11% inhibition of reduction at 10 mM | Malus domestica | |
| iodoacetate | 42% inhibition of oxidation and 19% inhibition of reduction at 10 mM | Malus domestica | |
| Na2EDTA | 35% inhibition of oxidation and 11% inhibition of reduction at 10 mM | Malus domestica | |
| p-chloromercuribenzoate | complete inhibition at 1.0 mM | Malus domestica | |
| ZnSO4 | complete inhibition of oxidation and 80% inhibition of reduction at 1.25 mM | Malus domestica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
D-glucose 6-phosphate | - |
Malus domestica | |
| 3.9 | - |
D-sorbitol 6-phosphate | - |
Malus domestica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Prunus armeniaca | important role in sorbitol synthesis | ? | - |
? | |
| additional information | Prunus persica | important role in sorbitol synthesis | ? | - |
? | |
| additional information | Malus domestica | important role in sorbitol synthesis | ? | - |
? | |
| additional information | Pyrus communis | important role in sorbitol synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
apple, cv. Starkrimson | - |
| Malus domestica | - |
cv. Antonovka | - |
| Malus domestica | - |
cv. Golden Delicious | - |
| Prunus armeniaca | - |
apricot, cv. Perfection | - |
| Prunus persica | - |
peach, cv. Redhaven | - |
| Pyrus communis | - |
pear, cv. Conference, cv. Bartlett | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | photosynthetically active tissue | Prunus armeniaca | - |
| leaf | photosynthetically active tissue | Prunus persica | - |
| leaf | photosynthetically active tissue | Malus domestica | - |
| leaf | photosynthetically active tissue | Pyrus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
measurement during development | Malus domestica |
| additional information | - |
distribution in Rosaceae species | Prunus armeniaca |
| additional information | - |
distribution in Rosaceae species | Prunus persica |
| additional information | - |
distribution in Rosaceae species | Malus domestica |
| additional information | - |
distribution in Rosaceae species | Pyrus communis |
| 628 | - |
- |
Malus domestica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 1°C, 1 week, partially purified enzyme | Malus domestica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose 6-phosphate + NADPH | reduced at low rate | Malus domestica | ? | - |
? | |
| D-galactose 6-phosphate + NADPH | reduced at a higher rate than D-glucose 6-phosphate | Malus domestica | ? | - |
r | |
| D-mannose 6-phosphate + NADPH | reduced at low rate | Malus domestica | ? | - |
? | |
| D-sorbitol 6-phosphate + NADP+ | - |
Prunus armeniaca | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | - |
Prunus persica | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | - |
Malus domestica | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | - |
Pyrus communis | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| additional information | not: D-glucose 1-phosphate, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose, sorbitol | Malus domestica | ? | - |
? | |
| additional information | important role in sorbitol synthesis | Prunus armeniaca | ? | - |
? | |
| additional information | important role in sorbitol synthesis | Prunus persica | ? | - |
? | |
| additional information | important role in sorbitol synthesis | Malus domestica | ? | - |
? | |
| additional information | important role in sorbitol synthesis | Pyrus communis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | D-sorbitol 6-phosphate oxidation | Malus domestica |
| 35 | - |
D-glucose 6-phosphate reduction | Malus domestica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no activity at 50°C | Malus domestica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
both directions | Malus domestica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10.1 | about 50% of activity maximum at pH 8.5 and 10.1 | Malus domestica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD+ or NADH | Malus domestica | |
| NADP+ | - |
Malus domestica | |
| NADPH | - |
Malus domestica | |
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