KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADPH | pH 8.0, 80°C | Thermococcus paralvinellae | |
0.05 | - |
2-phenylacetaldehyde | pH 8.0, 80°C | Thermococcus paralvinellae | |
0.25 | - |
acetaldehyde | pH 8.0, 80°C | Thermococcus paralvinellae | |
6.3 | - |
NADP+ | pH 8.0, 80°C | Thermococcus paralvinellae | |
8 | - |
ethanol | pH 8.0, 80°C | Thermococcus paralvinellae | |
15 | - |
2-phenylethanol | pH 8.0, 80°C | Thermococcus paralvinellae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thermococcus paralvinellae | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains 1 g-atom of Fe per subunit, present as a mixture of ferrous and ferric forms | Thermococcus paralvinellae | |
additional information | except for iron, no other metals are detected | Thermococcus paralvinellae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel filtration | Thermococcus paralvinellae |
46000 | - |
4 * 46000, SDS-PAGE | Thermococcus paralvinellae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus paralvinellae | - |
- |
- |
Thermococcus paralvinellae ES-1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NADP+ | 130% of the activity with ethanol | Thermococcus paralvinellae | butanal + NADPH + H+ | - |
r | |
1-butanol + NADP+ | 130% of the activity with ethanol | Thermococcus paralvinellae ES-1 | butanal + NADPH + H+ | - |
r | |
1-heptanol + NADP+ | 114% of the activity with ethanol | Thermococcus paralvinellae | heptanal + NADPH + H+ | - |
r | |
1-hexanol + NADP+ | 165% of the activity with ethanol | Thermococcus paralvinellae | hexanal + NADPH + H+ | - |
r | |
1-pentanol + NADP+ | 135% of the activity with ethanol | Thermococcus paralvinellae | pentanal + NADPH + H+ | - |
r | |
1-propanol + NADP+ | 102% of the activity with ethanol | Thermococcus paralvinellae | propanal + NADPH + H+ | - |
r | |
2-phenylacetaldehyde + NADPH + H+ | - |
Thermococcus paralvinellae | 2-phenylethanol + NADP+ | - |
r | |
2-phenylethanol + NADP+ | 70% of the activity with ethanol | Thermococcus paralvinellae | 2-phenylacetaldehyde + NADPH + H+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Thermococcus paralvinellae | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Thermococcus paralvinellae ES-1 | ethanol + NADP+ | - |
r | |
ethanol + NADP+ | - |
Thermococcus paralvinellae | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermococcus paralvinellae ES-1 | acetaldehyde + NADPH + H+ | - |
r | |
isobutanol + NADP+ | 93% of the activity with ethanol | Thermococcus paralvinellae | isobutanal + NADPH + H+ | - |
r | |
isobutanol + NADP+ | 93% of the activity with ethanol | Thermococcus paralvinellae ES-1 | isobutanal + NADPH + H+ | - |
r | |
additional information | enzyme utilizes NADP(H) as a cofactor and preferentially catalyzes aldehyde reduction | Thermococcus paralvinellae | ? | - |
? | |
additional information | enzyme utilizes NADP(H) as a cofactor and preferentially catalyzes aldehyde reduction | Thermococcus paralvinellae ES-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 46000, SDS-PAGE | Thermococcus paralvinellae |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermococcus paralvinellae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life 85 h | Thermococcus paralvinellae |
95 | - |
half-life 4 h | Thermococcus paralvinellae |
105 | - |
melting temperature | Thermococcus paralvinellae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | about 0.1% of the activity with NADPH | Thermococcus paralvinellae | |
NADPH | - |
Thermococcus paralvinellae |
General Information | Comment | Organism |
---|---|---|
physiological function | the specific activities of ADH, formate benzyl viologen oxidoreductase, and hydrogenase dramatically increase in cells grown under sulfur limitation. This is accompanied by increased amounts of H2 and alcohol (ethanol and butanol). Under S0 limitation, ADH may reduce to alcohols the aldehydes that are generated by fermentation, thereby serving to dispose of excess reductant | Thermococcus paralvinellae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
2-phenylethanol | pH 8.0, 80°C | Thermococcus paralvinellae | |
6 | - |
ethanol | pH 8.0, 80°C | Thermococcus paralvinellae | |
76.4 | - |
acetaldehyde | pH 8.0, 80°C | Thermococcus paralvinellae | |
300 | - |
2-phenylacetaldehyde | pH 8.0, 80°C | Thermococcus paralvinellae | |
345 | - |
NADPH | pH 8.0, 80°C | Thermococcus paralvinellae | |
2990 | - |
NADP+ | pH 8.0, 80°C | Thermococcus paralvinellae |