Literature summary for 1.1.1.2 extracted from

Vitale, A.; Thorne, N.; Lovell, S.; Battaile, K.P.; Hu, X.; Shen, M.; D'Auria, S.; Auld, D.S.
Physicochemical characterization of a thermostable alcohol dehydrogenase from Pyrobaculum aerophilum (2013), PLoS One, 8, e63828.

Search for more literature for 1.1.1.2

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion plates at 20°C. Crystal structure and X-ray fluorescence of wild-type and cobalt-substituted enzyme. Cocrystal structure of the enzyme with NADPH and analysis of the putative active site	Pyrobaculum aerophilum

Inhibitors

Inhibitors	Comment	Organism	Structure
1-(2-(bis-(4-fluorophenyl)methoxy)ethyl)-4-(3-phenyl-2-propenyl)piperazine	i.e. GBR13069. 0.08 mM, 61% inhibition	Pyrobaculum aerophilum
1-(2-diphenylmethoxyethyl)-4-(3-phenyl-2-propenyl)-piperazine	GBR12783, i.e. 0.08 mM, 59% inhibition	Pyrobaculum aerophilum
flunarizine	0.08 mM, 54% inhibition	Pyrobaculum aerophilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme contains both structural and catalytic Zn2+	Pyrobaculum aerophilum

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum aerophilum	Q8ZUP0	-	-
Pyrobaculum aerophilum DSM 7523	Q8ZUP0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+	3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone	Pyrobaculum aerophilum	1,2,3,4-tetrahydronaphthalen-1-ol + NADP+	-	r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+	3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone	Pyrobaculum aerophilum DSM 7523	1,2,3,4-tetrahydronaphthalen-1-ol + NADP+	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pyrobaculum aerophilum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)