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Literature summary for 1.1.1.2 extracted from

  • Brisdelli, F.; Saliola, M.; Pascarella, S.; Luzi, C.; Franceschini, N.; Falcone, C.; Martini, F.; Bozzi, A.
    Kinetic properties of native and mutagenized isoforms of mitochondrial alcohol dehydrogenase III purified from Kluyveromyces lactis (2004), Biochimie, 86, 705-712.
    View publication on PubMed
Search for more literature for 1.1.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 NADH pH 8.0, 25°C, wild-type Kluyveromyces lactis
0.025
-
 NADH wild type enzyme, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.045
-
 NADH pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
0.045
-
 NADH mutant enzyme G225A, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.05
-
 NADPH pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
0.06
-
 NADH pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
0.06
-
 NADH mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.07
-
 NADH pH 8.0, 25°C, mutant A274F Kluyveromyces lactis
0.07
-
 NADH mutant enzyme A274F, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.21
-
 NADPH pH 8.0, 25°C, wild-type Kluyveromyces lactis
0.22
-
 acetaldehyde pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
0.37
-
 NADPH pH 8.0, 25°C, mutant A274F Kluyveromyces lactis
0.37
-
 NADPH pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
0.39
-
 NAD+ pH 8.0, 25°C, wild-type Kluyveromyces lactis
0.39
-
 NAD+ wild type enzyme, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.5
-
 ethanol pH 8.0, 25°C, wild-type Kluyveromyces lactis
0.57
-
 NAD+ pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
0.57
-
 NAD+ mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication Kluyveromyces lactis
0.74
-
 ethanol pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
0.85
-
 ethanol pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
0.98
-
 NAD+ pH 8.0, 25°C, mutant A274F Kluyveromyces lactis
0.98
-
 NAD+ mutant enzyme A274F, at 25°C, pH not specified in the publication Kluyveromyces lactis
1
-
 NAD+ pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
1
-
 NAD+ mutant enzyme G225A, at 25°C, pH not specified in the publication Kluyveromyces lactis
1.02
-
 ethanol pH 8.0, 25°C, mutant A274F Kluyveromyces lactis
1.58
-
 acetaldehyde pH 8.0, 25°C, mutant A274F Kluyveromyces lactis
2.1
-
 acetaldehyde pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
2.33
-
 acetaldehyde pH 8.0, 25°C, wild-type Kluyveromyces lactis
2.64
-
 NADP+ pH 8.0, 25°C, wild-type Kluyveromyces lactis
2.77
-
 NADP+ pH 8.0, 25°C, mutant G225A Kluyveromyces lactis
5.5
-
 NADP+ pH 8.0, 25°C, mutant G225A/A274F Kluyveromyces lactis
6.21
-
 NADP+ pH 8.0, 25°C, mutant A274F Kluyveromyces lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Kluyveromyces lactis 5739
-

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis P49384
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADH + H+
-
 Kluyveromyces lactis ethanol + NAD+
-
 r
acetaldehyde + NADPH + H+
-
 Kluyveromyces lactis ethanol + NADP+
-
 r
benzaldehyde + NADPH 8% of activity with acetaldehyde Kluyveromyces lactis benzyl alcohol + NADP+
-
 r
butanal + NADPH + H+ 47% of activity with acetaldehyde Kluyveromyces lactis 1-butanol + NADP+
-
 r
butanol + NADP+ 68% of activity with ethanol Kluyveromyces lactis butanal + NADPH
-
 r
cinnamaldehyde + NADPH 5% of activity with acetaldehyde Kluyveromyces lactis cinnamic alcohol + NADP+
-
 r
cyclohexancarbaldehye + NADPH 3% of activity with acetaldehyde Kluyveromyces lactis cyclohexanol + NADP+
-
 r
cyclohexanol + NADP+ 14% of activity with ethanol Kluyveromyces lactis cyclohexanone + NADPH + H+
-
 r
ethanol + NAD+
-
 Kluyveromyces lactis acetaldehyde + NADH + H+
-
 r
ethanol + NADP+
-
 Kluyveromyces lactis acetaldehyde + NADPH + H+
-
 r
formaldehyde + NADPH 6% of activity with acetaldehyde Kluyveromyces lactis methanol + NADP+
-
 ir
glutaraldehyde + NADPH 5% of activity with acetaldehyde Kluyveromyces lactis ? + NADP+
-
 r
hexanol + NADP+ 60% of activity with ethanol Kluyveromyces lactis hexanal + NADPH
-
 r
isoamyl alcohol + NADP+ 18% of activity with ethanol Kluyveromyces lactis 3-methylbutanol + NADPH
-
 r
isopentanol + NADP+ 9% of activity with ethanol Kluyveromyces lactis 3-methylbutanal + NADPH + H+
-
 r
isopropanol + NADP+ 2% of activity with ethanol Kluyveromyces lactis acetone + NADPH
-
 r
additional information no substrate: methanol, 2-mercaptoethanol Kluyveromyces lactis ?
-
 ?
pentanol + NADP+ 75% of activity with ethanol Kluyveromyces lactis pentanal + NADPH
-
 r
propanal + NADPH + H+ 49% of activity with acetaldehyde Kluyveromyces lactis 1-propanol + NADP+
-
 r
propanol + NADP+ 70% of activity with ethanol Kluyveromyces lactis propanal + NADPH
-
 r
salicylaldehyde + NADPH 1% of activity with acetaldehyde Kluyveromyces lactis salicylalcohol + NADP+
-
 r