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Literature summary for 1.1.1.195 extracted from

  • Rong, W.; Luo, M.; Shan, T.; Wei, X.; Du, L.; Xu, H.; Zhang, Z.
    A wheat cinnamyl alcohol dehydrogenase TaCAD12 contributes to host resistance to the sharp eyespot disease (2016), Front. Plant Sci., 7, 1723.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Triticum aestivum
gene TaCAD12, cloned from cv. CI12633, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme, overexpression of the enyzem and of GFP-tagged enzyme in Triticum aestivum cv. CI12633 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0289
-
 coniferyl aldehyde recombinant GST-tagged enzyme, pH and temperature not specified in the publication Triticum aestivum
0.0342
-
 sinapyl aldehyde recombinant GST-tagged enzyme, pH and temperature not specified in the publication Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Triticum aestivum 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ require, the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39430
-
-
 Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl alcohol + NADP+ Triticum aestivum
-
 coniferyl aldehyde + NADPH + H+
-
 r
sinapyl alcohol + NADP+ Triticum aestivum
-
 sinapyl aldehyde + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-
Triticum aestivum A0A1D6BJ11 sharp eyespot-resistant and -susceptive lines/cultivars
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Triticum aestivum
-
additional information transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines Triticum aestivum
-
stem transcriptional patterns of TaCAD12 in wheat stems after Rhizoctonia cerealis inoculation analyzed by quantitative RT-PCR, overview Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative enzyme activity in different sharp eyespot-resistant and -susceptive lines/cultivars Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferaldehyde + NADPH + H+
-
 Triticum aestivum coniferyl alcohol + NADP+
-
 ?
coniferyl alcohol + NADP+
-
 Triticum aestivum coniferyl aldehyde + NADPH + H+
-
 r
coniferyl aldehyde + NADPH + H+
-
 Triticum aestivum coniferyl alcohol + NADP+
-
 r
sinapyl alcohol + NADP+
-
 Triticum aestivum sinapyl aldehyde + NADPH + H+
-
 r
sinapyl aldehyde + NADPH + H+
-
 Triticum aestivum sinapyl alcohol + NADP+
-
 r
sinapylaldehyde + NADPH + H+
-
 Triticum aestivum sinapyl alcohol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 39430, sequence calculation Triticum aestivum
More the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) with one highly conserved motif (196GLGGLG201) in CAD. The motif GLGGLG participates in binding the diophosphate group of NADP+ Triticum aestivum

Synonyms

Synonyms Comment Organism
CAD
-
 Triticum aestivum
CAD12
-
 Triticum aestivum
cinnamyl alcohol dehydrogenase
-
 Triticum aestivum
TaCAD12
-
 Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
additional information the motif GLGGLG participates in binding the diophosphate group of NADP+ Triticum aestivum
NADP+
-
 Triticum aestivum
NADPH
-
 Triticum aestivum

pI Value

Organism Comment pI Value Maximum pI Value
Triticum aestivum sequence calculation
-
 7.39

Expression

Organism Comment Expression
Triticum aestivum the transcriptional levels of the enzyme in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines up
Triticum aestivum the enzyme is induced by infection with Rhizoctonia cerealis, a necrotrophic fungus that causes the sharp eyespot disease in wheat. Transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines up

General Information

General Information Comment Organism
evolution TaCAD12 belongs to IV group in CAD family, phylogenetic analysis Triticum aestivum
malfunction knock-down of TaCAD12 transcript significantly represses resistance of the gene-silenced wheat plants to sharp eyespot caused by Rhizoctonia cerealis, whereas TaCAD12 overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot. Certain defense genes (Defensin, PR10, PR17c, and Chitinase1) and monolignol biosynthesis-related genes (TaCAD1, TaCCR, and TaCOMT1) are upregulated in the TaCAD12-overexpressing wheat plants but downregulated in TaCAD12-silencing plants Triticum aestivum
physiological function enzyme overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot caused by the necrotrophic fungus Rhizoctonia cerealis Triticum aestivum
physiological function enzyme CAD plays a role in defense responses to necrotrophic or soil-borne pathogens in wheat Triticum aestivum