Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D43E | only trace activities in presence and absence of NADPH | Homo sapiens |
D43N | only trace activities in presence and absence of NADPH | Homo sapiens |
H110A | only trace activities in presence and absence of NADPH | Homo sapiens |
H110F | only trace activities in presence and absence of NADPH | Homo sapiens |
K77L | only trace activities in presence and absence of NADPH | Homo sapiens |
K77R | only trace activities in presence and absence of NADPH | Homo sapiens |
Y48F | 69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
prostaglandin H2 | pH 5.5, 37°C | Homo sapiens | |
0.033 | - |
prostaglandin H2 | pH 5.0, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15121 | - |
- |
Mus musculus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
pH 5.5, 37°C | Homo sapiens |
3.6 | - |
pH 5.0, 37°C | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzaldehyde + NADPH + H+ | - |
Mus musculus | (4-nitrophenyl)methanol + NADP+ | - |
? | |
4-nitrobenzaldehyde + NADPH + H+ | - |
Homo sapiens | (4-nitrophenyl)methanol + NADP+ | - |
? | |
additional information | in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.018 mM. This activity activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively | Mus musculus | ? | - |
? | |
additional information | in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.023 mM. This activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively | Homo sapiens | ? | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Mus musculus | 9alpha,11beta-prostaglandin F2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Homo sapiens | 9alpha,11beta-prostaglandin F2 + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Mus musculus |
5.5 | - |
- |
Homo sapiens |