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Literature summary for 1.1.1.187 extracted from
- Guanosine diphosphate-4-keto-D-rhamnose reductase. A non-stereoselective enzyme (1971), J. Biol. Chem., 246, 5868-5876.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP-alpha-D-mannose | competitive inhibitor | Pseudomonas sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
GDP-4-dehydro-6-deoxy-D-mannose | with NADPH as reductant | Pseudomonas sp. | |
| 0.027 | - |
NADH | - |
Pseudomonas sp. | |
| 0.028 | - |
NADPH | - |
Pseudomonas sp. | |
| 0.05 | - |
GDP-4-dehydro-6-deoxy-D-mannose | with NADH as reductant | Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | 70000 | gel filtration | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | Pseudomonas sp. | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | GDP-6-deoxy-D-mannose + NAD(P)+ | - |
? | |
| GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | Pseudomonas sp. | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | GDP-D-talometylose + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
soil bacterium GS, ATCC 19241 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| DTT reduces instability at room temperature from 51% to 35% | Pseudomonas sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, fractionation-, chromatography-techniques | Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.98 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, half-life: 5 months | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | Pseudomonas sp. | GDP-6-deoxy-D-mannose + NAD(P)+ | - |
? | |
| GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | Pseudomonas sp. | GDP-D-talometylose + NAD(P)+ | - |
? | |
| GDP-4-dehydro-alpha-D-rhamnose + NADH + H+ | - |
Pseudomonas sp. | GDP-alpha-D-rhamnose + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP KR reductase | - |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
loss of activity in 5 min | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | with NADPH as reductant | Pseudomonas sp. |
| 8 | - |
with NADH as reductant | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | works equally well compared to NADPH | Pseudomonas sp. | |
| NADPH | - |
Pseudomonas sp. | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.72 | - |
GDP-mannose | competitive inhibition | Pseudomonas sp. | |
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