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Literature summary for 1.1.1.184 extracted from
- Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol (2016), Microb. Cell Fact., 15, 191.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | coexpression with glucose dehydrogenase from Bacillus subtilis for NADPH regeneration in Escherichia coli and optimization of linker peptides used for the fusion expression of carbonyl reductase and glucose dehydrogenase. Up to 297.3 g/L (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with enantiopurity >99.9% ee is produced via reduction of 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h) | Lentilactobacillus kefiri |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lentilactobacillus kefiri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentilactobacillus kefiri | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5-bis(trifluoromethyl) acetophenone + NADPH + H+ | - |
Lentilactobacillus kefiri | (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol + NADP+ | - |
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