KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
3-glutathionyl-4-hydroxynonanal | pH 8.4, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 0.1 mM NADP+, 1.5 M NaCl, stable for at least 2 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-glutathionyl-4-hydroxynonanal + NADPH + H+ | - |
Homo sapiens | 3-glutathionyl-1,4-dihydroxynonane + NADP+ | - |
? | |
4-oxo-2-nonenal + NADPH + H+ | 9% of the activity with 9,10-phenanthrenequinone | Homo sapiens | ? + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
menadione + NADPH + H+ | 48% of the activity with 9,10-phenanthrenequinone | Homo sapiens | ? + NADP+ | - |
? | |
additional information | carbonyl reductase oxidizes the hydroxyl group of 3-glutathionyl-4-hydroxynonanal in its hemiacetal form, with the formation of the corresponding 3-glutathionyl nonanoic-delta-lactone. The enzyme is practically inactive toward trans-4-hydroxy-2-nonenal, buthionine sulfoximine, N-acetylcysteine | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.75 | - |
3-glutathionyl-4-hydroxynonanal | pH 8.4, 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH | Homo sapiens | |
NADPH | - |
Homo sapiens |