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Literature summary for 1.1.1.184 extracted from

  • Fukuda, Y.; Sakuraba, H.; Araki, T.; Ohshima, T.; Yoneda, K.
    Catalytic properties and crystal structure of thermostable NAD(P)H-dependent carbonyl reductase from the hyperthermophilic archaeon Aeropyrum pernix K1 (2016), Enzyme Microb. Technol., 91, 17-25.
    View publication on PubMed
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional strcuture determined by molecular replacement at a resolution of 2.09 A, in the presence of NADPH. Extensive formation of the intrasubunit ion pair networks, and the presence of the strong intersubunit interaction, is likely responsible for thermostability Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
 9,10-phenanthrenequinone pH 6.0, 50°C Aeropyrum pernix
0.116
-
 NADPH pH 6.0, 50°C Aeropyrum pernix
0.176
-
 p-toluquinone pH 6.0, 50°C Aeropyrum pernix
0.7
-
 ethyl 4-chloro-3-oxobutanoate pH 6.0, 50°C Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9Y8Y1 cf. EC 1.1.1.100
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9,10-phenanthrenequinone + NADPH + H+
-
 Aeropyrum pernix ? + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Aeropyrum pernix ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
isatin + NADPH + H+
-
 Aeropyrum pernix 3-hydroxy-2-oxoindole + NADP+
-
 ?
p-toluquinone + NADPH
-
 Aeropyrum pernix ? + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 above 70°C Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 10 min, 75% residual activity Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 ethyl 4-chloro-3-oxobutanoate pH 6.0, 50°C Aeropyrum pernix
1.27
-
 9,10-phenanthrenequinone pH 6.0, 50°C Aeropyrum pernix
2.5
-
 p-toluquinone pH 6.0, 50°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aeropyrum pernix

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11.5
-
 Aeropyrum pernix