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Literature summary for 1.1.1.184 extracted from

  • Ni, Y.; Li, C.X.; Ma, H.M.; Zhang, J.; Xu, J.H.
    Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity (2011), Appl. Microbiol. Biotechnol., 89, 1111-1118.
    View publication on PubMed
Search for more literature for 1.1.1.184

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, 3.2% residual activity Bacillus sp. (in: Bacteria)
Cu2+ 1 mM, 7.2% residual activity Bacillus sp. (in: Bacteria)
Fe3+ 1 mM, no residual activity Bacillus sp. (in: Bacteria)
Ni2+ 1 mM, 4.8% residual activity Bacillus sp. (in: Bacteria)
Pb2+ 1 mM, 3.2% residual activity Bacillus sp. (in: Bacteria)
Zn2+ 1 mM, 2.4% residual activity Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) E3W861 putative
-
Bacillus sp. (in: Bacteria) ECU0013 E3W861 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2,2-trifluoroacetophenone + NADPH + H+ 140% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) (R)-2,2,2-trifluoro-1-phenylethanol + NADP+ 100% conversion, 99% enantiomeric excess ?
2,2,2-trifluoroacetophenone + NADPH + H+ 140% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ECU0013 (R)-2,2,2-trifluoro-1-phenylethanol + NADP+ 100% conversion, 99% enantiomeric excess ?
2-chloroacetophenone + NADPH + H+
-
 Bacillus sp. (in: Bacteria) (R)-2-chloro-1-phenylethanol + NADP+ 74.5% conversion, 99% enantiomeric excess ?
2-chloroacetophenone + NADPH + H+
-
 Bacillus sp. (in: Bacteria) ECU0013 (R)-2-chloro-1-phenylethanol + NADP+ 74.5% conversion, 99% enantiomeric excess ?
4'-chloroacetophenone + NADPH + H+ 64% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) (S)-1-(4-chlorophenyl)ethanol + NADP+ 49.6% conversion, 99% enantiomeric excess ?
4'-chloroacetophenone + NADPH + H+ 64% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ECU0013 (S)-1-(4-chlorophenyl)ethanol + NADP+ 49.6% conversion, 99% enantiomeric excess ?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+ 576% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+ 100% conversion, 85.7% enantiomeric excess ?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+ 576% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ECU0013 ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+ 100% conversion, 85.7% enantiomeric excess ?
ethyl 3-oxobutanoate + NADPH + H+ 145% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ethyl (3S)-3-hydroxybutanoate + NADP+ 100% conversion, 99% enantiomeric excess ?
ethyl 3-oxobutanoate + NADPH + H+ 145% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ECU0013 ethyl (3S)-3-hydroxybutanoate + NADP+ 100% conversion, 99% enantiomeric excess ?
ethyl 4,4,4-trifluoro-3-oxobutanoate + NADPH + H+ 74% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ethyl (3R)-4,4,4-trifluoro-3-hydroxybutanoate + NADP+ 100% conversion, 99% enantiomeric excess ?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ 8776% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ethyl (3R)-4-chloro-3-hydroxybutanoate + NADP+ 100% conversion, 62% enantiomeric excess ?
ethyl pyruvate + NADPH + H+ 7821% of the activity with 2-chloroacetophenone Bacillus sp. (in: Bacteria) ethyl (2S)-2-hydroxypropanoate + NADP+ 100% conversion, 99% enantiomeric excess ?

Synonyms

Synonyms Comment Organism
YtbE
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus sp. (in: Bacteria)