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Literature summary for 1.1.1.184 extracted from

  • Usami, N.; Ishikura, S.; Abe, H.; Nagano, M.; Uebuchi, M.; Kuniyasu, A.; Otagiri, M.; Nakayama, H.; Imamura, Y.; Hara, A.
    Cloning, expression and tissue distribution of a tetrameric form of pig carbonyl reductase (2003), Chem. Biol. Interact., 143-144, 353-361.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
genistein 50% inhibition at 0.028 mM Sus scrofa
kaempferol 50% inhibition at 0.015 mM Sus scrofa
myristic acid 50% inhibition at 0.025 mM Sus scrofa
quercetin 50% inhibition at 0.016 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
9,10-phenanthrenequinone pH 6.0, recombinant enzyme Sus scrofa
0.004
-
1-phenylisatin pH 6.0, recombinant enzyme Sus scrofa
0.021
-
hexanophenone pH 6.0, recombinant enzyme Sus scrofa
0.028
-
heptanophenone pH 6.0, recombinant enzyme Sus scrofa
0.03
-
isatin pH 6.0, recombinant enzyme Sus scrofa
0.031
-
nonanophenone pH 6.0, recombinant enzyme Sus scrofa
0.04
-
1-phenyl-1,2-propanedione pH 6.0, recombinant enzyme Sus scrofa
0.052
-
valerophenone pH 6.0, recombinant enzyme Sus scrofa
0.086
-
16-ketoestrone pH 6.0, recombinant enzyme Sus scrofa
0.09
-
4-hexanoylpyridine pH 6.0, recombinant enzyme Sus scrofa
0.13
-
benzil pH 6.0, recombinant enzyme Sus scrofa
0.17
-
n-butyrophenone pH 6.0, recombinant enzyme Sus scrofa
0.29
-
menadione pH 6.0, recombinant enzyme Sus scrofa
0.29
-
4-benzoylpyridine pH 6.0, recombinant enzyme Sus scrofa
0.47
-
2,3-hexandione pH 6.0, recombinant enzyme Sus scrofa
0.76
-
3-benzoylpyridine pH 6.0, recombinant enzyme Sus scrofa
0.89
-
propiophenone pH 6.0, recombinant enzyme Sus scrofa
1.4
-
Pyridine-4-aldehyde pH 6.0, recombinant enzyme Sus scrofa
4.5
-
diacetyl pH 6.0, recombinant enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000, SDS-PAGE Sus scrofa
100000
-
about, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart high expression level Sus scrofa
-
intestine
-
Sus scrofa
-
kidney high expression level Sus scrofa
-
liver
-
Sus scrofa
-
additional information tissue distribution, overview Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
purified recombinant enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenyl-1,2-propanedione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
?
1-phenylisatin + NADPH high activity, recombinant enzyme Sus scrofa ? + NADP+
-
?
16-ketoestrone + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
r
2,3-hexadione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
?
3-benzoylpyridine + NADPH recombinant enzyme Sus scrofa (S)-alpha-phenyl-3-pyridylmethanol + NADP+
-
?
4-benzoylpyridine + NADPH + H+ recombinant enzyme Sus scrofa (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
?
4-hexanoylpyridine + NADPH + H+ recombinant enzyme Sus scrofa 1-(pyridin-4-yloxy)hexan-1-ol + NADP+
-
?
9,10-phenanthrenequinone + NADPH best substrate, recombinant enzyme Sus scrofa ? + NADP+
-
r
9-cis-retinal + NADPH recombinant enzyme Sus scrofa 9-cis-retinol + NADP+
-
?
all-trans-retinal + NADPH + H+ recombinant enzyme Sus scrofa all-trans-retinol + NADP+
-
?
benzil + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
?
diacetyl + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
?
heptanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylheptan-1-ol + NADP+
-
?
hexanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylhexan-1-ol + NADP+
-
?
isatin + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
r
menadione + NADPH recombinant enzyme Sus scrofa ? + NADP+
-
?
additional information substrate specificity, overview Sus scrofa ?
-
?
n-butyrophenone + NADPH recombinant enzyme Sus scrofa 1-phenylbutan-1-ol + NADP+
-
?
nonanophenone + NADPH recombinant enzyme Sus scrofa 1-phenylnonan-1-ol + NADP+
-
?
propiophenone + NADPH recombinant enzyme Sus scrofa 1-phenylpropan-1-ol + NADP+
-
?
pyridine-4-aldehyde + NADPH + H+ recombinant enzyme Sus scrofa pyridin-4-ylmethanol + NADP+
-
?
valerophenone + NADPH recombinant enzyme Sus scrofa 1-phenylpentan-1-ol + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
carbonyl reductase
-
Sus scrofa
CR
-
Sus scrofa
PHCR
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
about Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sus scrofa