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Literature summary for 1.1.1.184 extracted from

  • Usui, S.; Hara, A.; Nakayama, T.; Sawada, H.
    Purification and characterization of two forms of microsomal carbonyl reductase in guinea pig liver (1984), Biochem. J., 223, 697-705.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
21-Hydroxy-5alpha-pregnane-3,20-dione
-
Cavia porcellus
3,3-Tetramethyleneglutaric acid enzyme form CR2 inhibited, CR1 slightly Cavia porcellus
5beta-androstane-3alpha,17beta-diol
-
Cavia porcellus
5beta-pregnane-3,20-dione
-
Cavia porcellus
AgNO3 enzyme form CR1 inhibited, CR2 not Cavia porcellus
Ammonium molybdate enzyme form CR1 inhibited, CR2 not Cavia porcellus
androsterone CR2 inhibited, CR1 not Cavia porcellus
chlorpromazine
-
Cavia porcellus
dicoumarol enzyme form CR2 inhibited, CR1 not Cavia porcellus
Hg2+
-
Cavia porcellus
indomethacin
-
Cavia porcellus
p-chloromercuribenzoate
-
Cavia porcellus
progesterone
-
Cavia porcellus
quercitrin
-
Cavia porcellus
Sodium phenobarbitone enzyme form CR2 inhibited, CR1 slightly Cavia porcellus
testosterone
-
Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information overview Cavia porcellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, enzyme form CR2, SDS-PAGE Cavia porcellus
34000
-
gel filtration, enzyme form CR1 Cavia porcellus
37500
-
1 * 37500, enzyme form CR1, SDS-PAGE Cavia porcellus
115000
-
gel filtration, enzyme form CR2 Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 enzyme forms: major form CR2, minor form CR1 Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrobenzaldehyde + NADPH
-
Cavia porcellus 2-nitrobenzyl alcohol + NADP+
-
?
3-nitroacetophenone + NADPH
-
Cavia porcellus ?
-
?
3-nitrobenzaldehyde + NADPH
-
Cavia porcellus 3-nitrobenzyl alcohol + NADP+
-
?
4-nitroacetophenone + NADPH
-
Cavia porcellus ?
-
?
4-nitrobenzaldehyde + NADPH
-
Cavia porcellus 4-nitrobenzyl alcohol + NADP+
-
?
benzaldehyde + NADPH
-
Cavia porcellus benzyl alcohol + NADP+
-
?
diacetyl + NADPH
-
Cavia porcellus ?
-
?
additional information overview Cavia porcellus ?
-
?
additional information specificity of various enzyme forms Cavia porcellus ?
-
?
phenylglyoxal + NADPH
-
Cavia porcellus ?
-
?
pyridine-4-aldehyde + NADPH
-
Cavia porcellus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37500, enzyme form CR1, SDS-PAGE Cavia porcellus
tetramer 4 * 32000, enzyme form CR2, SDS-PAGE Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8 5.3 4-nitroacetophenone, pyridine 4-aldehyde, 5beta-dihydrotestosterone, enzyme CR2 Cavia porcellus
5.8 6.2 4-nitroacetophenone, pyridine 4-aldehyde, enzyme form CR1 Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Cavia porcellus
NADPH
-
Cavia porcellus