Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis | Perilla frutescens |
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis | Perilla frutescens var. hirtella |
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Perilla frutescens var. hirtella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + NADP+ | Perilla frutescens | - |
geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | Perilla frutescens var. hirtella | - |
geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | Perilla frutescens var. hirtella | - |
geranial + NADPH + H+ | - |
r | |
geraniol + NADP+ | Perilla frutescens 32 | - |
geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | Perilla frutescens var. hirtella 5031 | - |
geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | Perilla frutescens var. hirtella 87 | - |
geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | Perilla frutescens var. hirtella 87 | - |
geranial + NADPH + H+ | - |
r | |
additional information | Perilla frutescens var. hirtella | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | ? | - |
? | |
additional information | Perilla frutescens | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | ? | - |
? | |
additional information | Perilla frutescens 32 | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | ? | - |
? | |
additional information | Perilla frutescens var. hirtella 87 | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Perilla frutescens | K9S1J6 | - |
- |
Perilla frutescens | K9S1J6 | PA type | - |
Perilla frutescens 32 | K9S1J6 | PA type | - |
Perilla frutescens var. hirtella | - |
- |
- |
Perilla frutescens var. hirtella | K9RZL7 | - |
- |
Perilla frutescens var. hirtella | K9RZL7 | C type | - |
Perilla frutescens var. hirtella | K9S0X0 | - |
- |
Perilla frutescens var. hirtella | K9S0X0 | SF type | - |
Perilla frutescens var. hirtella 5031 | K9S0X0 | SF type | - |
Perilla frutescens var. hirtella 87 | K9RZL7 | C type | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS | Perilla frutescens var. hirtella |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Perilla frutescens | - |
leaf | - |
Perilla frutescens var. hirtella | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella | geraniol + NADP+ | - |
r | |
geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella 87 | geraniol + NADP+ | - |
r | |
geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella | nerol + NADP+ | PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry | r | |
geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella 87 | nerol + NADP+ | PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry | r | |
geraniol + NADP+ | - |
Perilla frutescens | geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | - |
Perilla frutescens var. hirtella | geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | - |
Perilla frutescens var. hirtella | geranial + NADPH + H+ | - |
r | |
geraniol + NADP+ | - |
Perilla frutescens 32 | geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | - |
Perilla frutescens var. hirtella 5031 | geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | - |
Perilla frutescens var. hirtella 87 | geranial + NADPH + H+ | - |
? | |
geraniol + NADP+ | - |
Perilla frutescens var. hirtella 87 | geranial + NADPH + H+ | - |
r | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | Perilla frutescens var. hirtella | ? | - |
? | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens | ? | - |
? | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens var. hirtella | ? | - |
? | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens 32 | ? | - |
? | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens var. hirtella 5031 | ? | - |
? | |
additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | Perilla frutescens var. hirtella 87 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GeDH | - |
Perilla frutescens |
GeDH | - |
Perilla frutescens var. hirtella |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Perilla frutescens |
30 | - |
assay at | Perilla frutescens var. hirtella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Perilla frutescens |
7 | - |
assay at | Perilla frutescens var. hirtella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Perilla frutescens | |
NADP+ | - |
Perilla frutescens var. hirtella |
General Information | Comment | Organism |
---|---|---|
evolution | GeDH gene expression in C-, PA-, PK-, and PP-type Perilla | Perilla frutescens |
evolution | GeDH gene expression in C-, PA-, PK-, and PP-type Perilla | Perilla frutescens var. hirtella |
metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil | Perilla frutescens |
metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil | Perilla frutescens var. hirtella |
metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil. Geranial is formed from geraniol by enzymatic reaction of Perilla aldo-keto reductase or Perilla GeDH | Perilla frutescens var. hirtella |
physiological function | two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde | Perilla frutescens |
physiological function | two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde | Perilla frutescens var. hirtella |