Cloned (Comment) | Organism |
---|---|
expression of His-tagged native and selenomethionine-labeled enzyme in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged native and selenomethionine-labeled enzyme, 12 mg/ml wild-type protein in 25 mM Tris, pH 8.0, and 13.8 mg/ml of selenomethionine-labeled enzyme in 20 mM Tris, pH 8.0, and 5 mM DTT, 4°C, modified microbatch method, equal volumes of protein solution and precipitant solution of 0.001 ml, the latter containing 20%w/v PEG 3350, 0.20 M potassium fluoride, pH 8.5-9.0, are mixed, overlaid with a 1:1 mixture of silicone and paraffin oils, X-ray anomalous diffraction structure determination and analysis at 1.75-2.0 A resolution, molecular replacement | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39170 | - |
x * 39170, His-tagged recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Bacillus subtilis | first enzyme in the catabolic pathway of myo-inositol | scyllo-inosose + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged native and selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography to homogeneity | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | His176 and Asp172 are proposed to act as the catalytic dyad, in which His176 is proposed to be the acid/base catalyst | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | first enzyme in the catabolic pathway of myo-inositol | Bacillus subtilis | scyllo-inosose + NADH | - |
r | |
myo-inositol + NAD+ | oxidation of the axial hydroxyl group of myo-inositol | Bacillus subtilis | scyllo-inosose + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 39170, His-tagged recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Bacillus subtilis |
inositol dehydrogenase | - |
Bacillus subtilis |
More | IDH is a member of the GFOR/IDH/MocA family | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NADH | - |
Bacillus subtilis |