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Literature summary for 1.1.1.175 extracted from

  • Toivari, M.; Vehkomaeki, M.; Nygard, Y.; Penttilae, M.; Ruohonen, L.; Wiebe, M.
    Low pH D-xylonate production with Pichia kudriavzevii (2013), Biores. Technol., 133, 555-562.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xylB, recombinnat expression in Pichia kudriavzevii strain VTT C-79090T Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
additional information engineering of non-conventional yeast Pichia kudriavzevii strain VTT C-79090T to recombinantly express the D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant strain VTT C-79090T produces up to 171 g/l of D-xylonate from 171 g/l D-xylose at a rate of 1.4 g/l/h and a high yield, which is comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain is also remarkably high at low pH, producing 146 g/l D-xylonate at pH 3.0. Comparison to production of D-xylonate from Saccharomyces cerevisiae strain VTT B-67002 expressing the gene from Caulobacter crescentus, overview Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Caulobacter vibrioides
-
D-xylonolactone + NADH + H+
-
?
D-xylose + NAD+ Caulobacter vibrioides NA1000
-
D-xylonolactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides B8H1Z0
-
-
Caulobacter vibrioides NA1000 B8H1Z0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+
-
Caulobacter vibrioides D-xylonolactone + NADH + H+
-
?
D-xylose + NAD+
-
Caulobacter vibrioides NA1000 D-xylonolactone + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
D-xylose 1-dehydrogenase
-
Caulobacter vibrioides
XylB
-
Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Caulobacter vibrioides